AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging
AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging
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DOI：
10.1007/s11307-010-0299-8
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发表时间：
2010-10-01
影响因子：
3.1
通讯作者：
Redmond, Peter
中科院分区：
文献类型：
--
作者：
Aydogan, Bulent;Li, Ji;Redmond, Peter
为了通过体外实验研究2-脱氧葡萄糖(2-DG)标记的金纳米颗粒(AuNP-DG)作为具有肿瘤靶向性的CT造影剂的可行性。选择人肺泡上皮癌细胞株A-549进行体外细胞摄取实验。两组细胞样本分别与AuNP-DG和未标记的AuNP孵育。孵育后，用无菌PBS清洗细胞，以去除多余的金纳米颗粒，并使用离心机旋转成细胞球。离心后立即使用MicroCT扫描仪对细胞颗粒进行成像。用商业软件对重建的CT图像进行分析。在多个CT切片上，观察到与AuNP-DG孵育的细胞样本相比与与未标记的AuNP孵育的细胞样本相比，显著的对比增强。本研究结果表明，肿瘤细胞在体外增强了2-DG标记的金纳米颗粒的摄取，这为进一步实验研究AuNP-DG内化并保留在肿瘤细胞中的确切分子机制奠定了基础。
To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments.Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package.Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices.Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.