Diversity and succession of the intestinal bacterial community of the maturing broiler chicken
Diversity and succession of the intestinal bacterial community of the maturing broiler chicken
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DOI：
10.1128/aem.69.11.6816-6824.2003
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发表时间：
2003-11-01
影响因子：
4.4
通讯作者：
Lee, MD
中科院分区：
文献类型：
--
作者：
Lu, JR;Idris, U;Lee, MD
通过对1230个16S rRNA基因部分序列的分析，研究了饲喂不含饲料添加剂的玉米-大豆混合素食肉鸡的肠段和盲肠细菌区系的多样性。回肠中近70%的序列与乳杆菌有关，其余大部分与梭菌科(11%)、链球菌(6.5%)和肠球菌(6.5%)有关。相反，在盲肠中检测到的与梭菌科相关的序列(65%)最丰富，其他最丰富的序列与梭杆菌(14%)、乳杆菌(8%)和类杆菌(5%)有关。对不同16S rRNA文库组成的统计分析表明，种群演替发生在一定的采样时期。3日龄、7日龄、14~28日龄和49日龄盲肠库之间的显著差异表明，随着年龄的增长，盲肠库发生了从短暂群落到日益复杂的群落的演替。在7日龄至21日龄和21日龄至28日龄之间，回肠具有稳定的细菌群落结构，但在3日龄和49日龄时，回肠具有非常独特的群落结构。在3日龄时，回肠和盲肠库的组成没有显著差异，事实上，在前14天，盲肠微生物区系是回肠微生物区系的一个亚群。此后，回肠和盲肠具有显著不同的文库组成，这表明随着鸟类的成熟，每个区域都发展了自己独特的细菌群落。
The diversity of bacterial floras in the ilea and ceca of chickens that were fed a vegetarian corn-soy broiler diet devoid of feed additives was examined by analysis of 1,230 partial 16S rRNA gene sequences. Nearly 70% of sequences from the ileum were related to those of Lactobacillus, with the majority of the rest being related to Clostridiaceae (11%), Streptococcus (6.5%), and Enterococcus (6.5%). In contrast, Clostridiaceae-related sequences (65%) were the most abundant group detected in the cecum, with the other most abundant sequences being related to Fusobacterium (14%), Lactobacillus (8%), and Bacteroides (5%). Statistical analysis comparing the compositions of the different 16S rRNA libraries revealed that population succession occurred during some sampling periods. The significant differences among cecal libraries at 3 and 7 days of age, at 14 to 28 days of age, and at 49 days of age indicated that successions occurred from a transient community to one of increasing complexity as the birds aged. Similarly, the ileum had a stable bacterial community structure for birds at 7 to 21 days of age and between 21 to 28 days of age, but there was a very unique community structure at 3 and 49 days of age. It was also revealed that the composition of the ileal and cecal libraries did not significantly differ when the birds were 3 days old, and in fact during the first 14 days of age, the cecal microflora was a subset of the ileal microflora. After this time, the ileum and cecum had significantly different library compositions, suggesting that each region developed its own unique bacterial community as the bird matured.