Impact of myocardial contrast echocardiography on vascular permeability: Comparison of three different contrast agents
Impact of myocardial contrast echocardiography on vascular permeability: Comparison of three different contrast agents
复制标题
DOI：
10.1016/j.ultrasmedbio.2003.09.004
复制
发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Miller, DL
中科院分区：
文献类型：
--
作者：
Li, P;Armstrong, WF;Miller, DL
微血管通透性、点状出血和室性早搏 (PVC) 已在心肌造影超声心动图 (MCE) 的体内大鼠模型中得到证实。本研究的目的是比较三种美国食品和药物管理局 (FDA) 批准的超声 (US) 造影剂 (US CA) 的效果：Optison(R)、Definity(R) 和 Imagent(R)。伊文思蓝染料（微血管通透性指标）和造影剂被静脉注射到悬浮在水浴中的麻醉大鼠中，以模拟临床超声心动图所见的扫描深度。使用心脏相控阵扫描头以 1.7 MHz 进行 1:4 收缩末期触发的诊断性 US B 模式扫描，以提供左心室的短轴视图。为了获得易于测量的比较效果，使用了相对较高剂量的试剂（Optison(R) 为 50 至 500 µL kg(-1)，Imagent(R) 为 25 至 200 µL kg(-1)，Definity(R) 为 10 至 100 µL kg(-1)）。微血管渗漏的特征是通过心脏上伊文思蓝染料着色的面积以及从组织样本中提取染料来表征。计数离体心脏心外膜表面的瘀点数量。 PVC 根据 MCE 图像记录的 ECG 迹线进行计数。在假手术动物中没有发现毛细血管渗漏或 PVC 的证据。根据体积剂量，Definity(R) MCE 产生更多的微血管渗漏，但三种药物的微血管损伤潜力之间没有明显差异，当以稳定微泡的数量表示时，微血管损伤潜力在低剂量下随剂量线性增加。 Definity(R) MCE 比其他试剂产生更少的 PVC。这种影响随着峰值稀疏压力幅度的增加而强烈增加，瘀点的明显阈值为 0.4 MPa，PVC 的明显阈值约为 1.0 MPa。这些结果对于最大限度地减少诊断中的潜在不利因素和优化治疗应用中的功效应该具有价值。 (C) 2004 年世界超声医学生物学联合会。
Microvascular permeabilization, petechial hemorrhage and premature ventricular contractions (PVCs) have been demonstrated in an in vivo rat model of myocardial contrast echocardiography (MCE). The purpose of this study was to compare these effects for three US Food and Drug Administration (FDA)-approved ultrasound (US) contrast agents (US CA): Optison(R), Definity(R) and Imagent(R). Evans blue dye, an indicator of microvascular permeability, and a contrast agent were injected IV in anesthetized rats suspended in a water bath to mimic scanning depths seen in clinical echocardiology. Diagnostic US B-mode scans with 1:4 end-systolic triggering were performed at 1.7 MHz using a cardiac phased-array scanhead to provide a short axis view of the left ventricle. To elicit readily measurable effects for comparisons, relatively high doses of the agent were used (50 to 500 muL kg(-1) for Optison(R), 25 to 200 muL kg(-1) for Imagent(R), 10 to 100 muL kg(-1) for Definity(R)). Microvascular leakage was characterized by the area of Evans blue dye coloration on the hearts and by extraction of the dye from tissue samples. The number of petechia were counted on the epicardial surface of excised hearts. PVCs were counted from ECG traces recorded with the MCE images. Neither evidence of capillary leakage nor PVCs were seen in sham animals. Based on volume dose, Definity(R) MCE produced more microvascular leakage, but there was no apparent difference between the three agents' microvascular damage potential, which increased linearly with dose at low doses, when expressed in terms of the number of stabilized microbubbles. Definity(R) MCE resulted in fewer PVCs than the other agents. The effects increased strongly with peak rarefactional pressure amplitude, with apparent thresholds for petechiae at 0.4 MPa and for PVCs at about 1.0 MPa. These results should be of value for minimizing adverse potential in diagnosis and optimizing efficacy in therapeutic applications. (C) 2004 World Federation for Ultrasound in Medicine Biology.