HIF-1α ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron
HIF-1α ODD polypeptides increased the expression of HIF1 and VEGF in hypoxic rat cortical neuron
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DOI：
10.1007/s10072-011-0574-8
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发表时间：
2011-12-01
影响因子：
3.3
通讯作者：
Xi, Qi
中科院分区：
文献类型：
--
作者：
Liu, Xuemin;Du, Juan;Xi, Qi
探讨缺氧诱导因子-1 α寡核苷酸多肽对缺氧原代培养大鼠皮层神经元缺氧诱导因子-1 α和血管内皮生长因子表达的影响。原代培养的神经元暴露于缺氧2，4，和8小时。实验组在缺氧前加入Tat-ODD融合多肽。Western blot和真实的PCR检测HIF-1 α和VEGF的表达。HIF-1 α和VEGF水平在缺氧4 h达到峰值，然后下降。tat-ODD融合多肽可增加HIF-1 α和VEGF的表达。tat-ODD融合多肽可能通过增加HIF-1 α和VEGF的表达来改善HIF 1对缺氧的适应。
To investigate the effect of HIF-1 alpha ODD polypeptides on the expression of HIF-1 alpha and VEGF in primary rat cortical neuron in hypoxia. Primary cultured neurons were exposed to hypoxia for 2, 4, and 8 h. Tat-ODD fusion polypeptide was added before hypoxia in the experimental groups. The expression of HIF-1 alpha and VEGF was detected by Western blot and real time PCR. The levels of HIF-1 alpha and VEGF peaked at 4 h then declined in hypoxia. The expression of HIF-1 alpha and VEGF was increased by tat-ODD fusion polypeptide. tat-ODD fusion polypeptide might improve HIF1 adaptation to hypoxia by increased expression of HIF-1 alpha and VEGF.