O-ACETYLATION OF DISIALOGANGLIOSIDE-GD3 BY HUMAN-MELANOMA CELLS CREATES A UNIQUE ANTIGENIC DETERMINANT
O-ACETYLATION OF DISIALOGANGLIOSIDE-GD3 BY HUMAN-MELANOMA CELLS CREATES A UNIQUE ANTIGENIC DETERMINANT
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DOI：
10.1126/science.6206564
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发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
VARKI, AP
中科院分区：
文献类型：
--
作者：
CHERESH, DA;REISFELD, RA;VARKI, AP
单抗Mab D1.1识别人类黑色素瘤细胞上的神经节苷脂抗原，其特征是具有碱性-O-乙酰化唾液酸残基。免疫化学分析表明，该分子是神经外胚层相关的二唾液酸神经节苷脂GD3的乙酰化产物。纯化的GD3的受控化学O-乙酰化导致了该相同表位的产生。人黑色素瘤细胞裂解产物含有O-乙酰转移酶活性，能够产生单抗D1.1识别的抗原表位。因此，在共同的细胞表面相关神经节苷脂上添加一个单一的O-乙酰基可以产生潜在的肿瘤特异性抗原。
Monoclonal antibody Mab D1.1 recognizes on human melanoma cells a ganglioside antigen characterized by an alkali-labileO-acetylated sialic acid residue. Immunochemical analysis showed that this molecule is anO-acetylated product of the neuroectoderm-associated disialoganglioside GD3. Controlled chemicalO-acetylation of purified GD3resulted in the generation of this same epitope. Lysates of human melanoma cells were found to containO-acetyltransferase activity capable of generating the antigenic epitope recognized by Mab D1.1. Thus, the addition of a singleO-acetyl group to a common cell surface-associated ganglioside can create a potentially tumor-specific antigen.