2 SIMPLE PROCEDURES FOR RELEASING CHROMATIN FROM ROUTINELY FIXED CELLS FOR FLUORESCENCE IN-SITU HYBRIDIZATION
2 SIMPLE PROCEDURES FOR RELEASING CHROMATIN FROM ROUTINELY FIXED CELLS FOR FLUORESCENCE IN-SITU HYBRIDIZATION
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DOI:
10.1159/000133632
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发表时间:
1994-01-01
期刊:
影响因子:
--
通讯作者:
SHEER, D
中科院分区:
文献类型:
--
作者:
FIDLEROVA, H;SENGER, G;SHEER, D
We describe two methods for releasing chromatin from routinely harvested and fixed cells. Using fluorescence in situ hybridization with combinations of probes from the HLA class II region, we show that good signals can be obtained on free chromatin fibers enabling determination of relationships between closely adjacent or overlapping probes.