Labeling and imaging mesenchymal stem cells with quantum dots.
Labeling and imaging mesenchymal stem cells with quantum dots.
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DOI：
10.1007/978-1-61779-953-2_15
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Muller-Borer, Barbara J
中科院分区：
文献类型：
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作者：
Collins, Maria C;Gunst, Philip R;Muller-Borer, Barbara J
骨髓间充质干细胞是一种具有向骨、软骨、脂肪和肌肉细胞分化潜能的多能细胞。成人来源的间充质干细胞正在积极研究，因为他们的潜力，可用于治疗性细胞为基础的移植。在体内追踪MSC的方法是有限的，阻止了移植细胞的长期功能研究。量子点（QD）为有机染料和荧光蛋白提供了一种替代方案，用于在体外和体内标记和跟踪细胞。纳米颗粒对化学和代谢降解具有抗性，表现出长期的光稳定性。在这里，我们描述的技术，标记的MSC与量子点，并证明量子点在标记的MSC与激光扫描共聚焦荧光显微镜细胞内的分布。
Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into bone, -cartilage, adipose, and muscle cells. Adult derived MSCs are being actively investigated because of their potential to be utilized for therapeutic cell-based transplantation. Methods to track MSCs in vivo are -limited, preventing long-term functional studies of transplanted cells. Quantum Dots (QDs) offer an alternative to organic dyes and fluorescent proteins to label and track cells in vitro and in vivo. Nanoparticles are resistant to chemical and metabolic degradation, demonstrating long-term photostability. Here, we describe the technique to label MSCs with QDs and demonstrate intracellular QD distribution in the labeled MSCs with laser scanning confocal fluorescent microscopy.