Differential effects of exercise training on skeletal muscle SERCA gene expression.
Differential effects of exercise training on skeletal muscle SERCA gene expression.
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运动训练对骨骼肌 SERCA 基因表达的不同影响。
DOI：
10.1097/00005768-200301000-00005
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发表时间：
2003
影响因子：
4.1
通讯作者：
Houser,StevenR
中科院分区：
文献类型：
--
作者：
Kubo,Hajime;Libonati,JosephR;Kendrick,ZebulonV;Paolone,Albert;Gaughan,JohmP;Houser,StevenR
久保，H., JR .利伯纳提，ZV肯德里克，A.保隆，JP高根，和SR豪瑟。运动训练对骨骼肌SERCA基因表达的差异影响。医学科学。体育Exerc。，第35卷第1期，第27-31页，2003。运动训练可引起骨骼肌表现的显著变化。目的：确定大鼠后肢肌浆网ca2 - atp酶亚型（SERCA1a和SERCA2a）基因表达在中、高强度运动训练中的变化是否为功能重塑奠定了基础。方法：用northern分析法测定以快肌、腓肠肌为主、慢肌、比目鱼肌为主的SERCA1a和SERCA2a亚型mRNA的表达。用同一样品中测得的18S rRNA将SERCA mRNA归一化。结果：中、高强度训练后腓肠肌SERCA2a mRNA表达均显著升高。在任何条件下，SERCA1a的表达均未发生显著变化。结论：中、高强度运动均可增加腓肠肌中SERCA2a的相对表达。关键词：NORTHERN BLOT， MRNA，
KUBO, H., JR LIBONATI, ZV KENDRICK, A. PAOLONE, JP GAUGHAN, and SR HOUSER. Differential Effects of Exercise Training on Skeletal Muscle SERCA Gene Expression. Med. Sci. Sports Exerc., Vol. 35, No. 1, pp. 27–31, 2003.Introduction: Exercise training induces significant changes in the performance of skeletal muscle. Purpose: To determine whether changes in the gene expression of rat hind-limb muscle sarcoplasmic reticulum Ca2-ATPase isoforms (SERCA1a and SERCA2a) in response to either moderate-or high-intensity exercise training underlie the functional remodeling. Methods: SERCA1a and SERCA2a isoform mRNA expression was determined in predominantly fast-twitch, gastrocnemius muscle and predominantly slow-twitch, soleus muscle with northern analysis. SERCA mRNA was normalized by the 18S rRNA measured in the same sample. Results: Significant increases in the gastrocnemius SERCA2a mRNA expression were observed after both moderate-and high-intensity training. No significant change in SERCA1a expression was found under any conditions. Conclusion: These results indicate that both moderate and high intensity exercise increase the relative SERCA2a expression in the gastrocnemius. Key Words: NORTHERN BLOT, MRNA,