The Use of Metabolomics to Identify Biological Signatures of Manganese Exposure
The Use of Metabolomics to Identify Biological Signatures of Manganese Exposure
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DOI：
10.1093/annweh/wxw032
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发表时间：
2017-05-01
影响因子：
2.6
通讯作者：
Seixas, Noah
中科院分区：
文献类型：
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作者：
Baker, Marissa G.;Simpson, Christopher D.;Seixas, Noah
目的：锰（Mn）是一种已知的神经毒物，鉴于其对健康的影响和在金属加工环境中普遍存在的性质，确定锰暴露的有效和可重复的生物标志物是有意义的。在这里，全球代谢组学是用来确定不同的代谢物组之间定义的锰暴露状态，目标是帮助通知锰exposition.Methods的潜在代谢物生物标志物：锰暴露的主题被招募从锰钢铁铸造和锰未暴露的主题被招募从起重机操作员在金属回收设施。在一个工作日的过程中，每个受试者佩戴个人可吸入粉尘采样器（IOM），并提供轮班结束时的尿液样本，进行整体代谢组学分析。暴露和未暴露受试者均被分为训练集和人口统计学相似的验证集。使用双侧调整的t检验，比较Mn暴露和未暴露训练集以及具有错误发现率（FDR）的训练集之间发现的所有代谢物的相对丰度。
Objectives: Manganese (Mn) is a known neurotoxicant, and given its health effects and ubiquitous nature in metal-working settings, identification of a valid and reproducible biomarker of Mn exposure is of interest. Here, global metabolomics is utilized to determine metabolites that differ between groups defined by Mn exposure status, with the goal being to help inform a potential metabolite biomarker of Mn exposure.Methods: Mn exposed subjects were recruited from a Mn steel foundry and Mn unexposed subjects were recruited from crane operators at a metal recycling facility. Over the course of a work day, each subject wore a personal inhalable dust sampler (IOM), and provided an end of shift urine sample that underwent global metabolomics profiling. Both exposed and unexposed subjects were divided into a training set and demographically similar validation set. Using a two-sided adjusted t-test, relative abundances of all metabolites found were compared between Mn exposed and unexposed training sets, and those with a false discovery rates (FDR)