Characteristics of the ATP-induced Ca2+-entry pathway in the t-BBEC 117 cell line derived from bovine brain endothelial cells.
Characteristics of the ATP-induced Ca2+-entry pathway in the t-BBEC 117 cell line derived from bovine brain endothelial cells.
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源自牛脑内皮细胞的 t-BBEC 117 细胞系中 ATP 诱导的 Ca2+ 进入途径的特征。
DOI：
10.1254/jphs.sc0070080
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发表时间：
2007
影响因子：
3.5
通讯作者：
Y. Imaizumi
中科院分区：
文献类型：
--
作者：
D. Yamazaki;S. Ohya;K. Asai;Y. Imaizumi
atp受体（P2Y）刺激诱导持续的Ca2+进入，这是增强t-BBEC117细胞增殖所必需的，t-BBEC117是来源于牛脑内皮细胞的永生化细胞系。在Ca2+无钙溶液中使用thapsigarin耗尽存储后，Ca2+的应用诱导Ca2+通过存储操作通道（soc）进入。ATP或1-油基-2-乙酰基-sn-甘油（OAG）与Ca2+共同诱导的Ca2+进入明显大于Ca2+单独诱导的Ca2+进入，表明受体操作通道（ROCs）参与Ca2+进入。使用药理学工具获得的结果表明，Ca2+来源对atp诱导的t-BBEC117中[Ca2+]i升高的贡献估计分别为通过soc和ROCs释放Ca2+和进入Ca2+的约2:1。
ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively.