ANTIGENIC RELATIONSHIP BETWEEN CHIKUNGUNYA VIRUS-STRAINS AND ONYONG NYONG VIRUS USING MONOCLONAL-ANTIBODIES
ANTIGENIC RELATIONSHIP BETWEEN CHIKUNGUNYA VIRUS-STRAINS AND ONYONG NYONG VIRUS USING MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0923-2516(96)80591-7
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
GIBSON, G
中科院分区：
文献类型：
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作者：
BLACKBURN, NK;BESSELAAR, TG;GIBSON, G
使用一组针对非洲菌株 H817 和亚洲菌株 PhH15483 制备的单克隆抗体 (mAb)，显示来自非洲和亚洲的基孔肯雅 (CHIK) 菌株在抗原上密切相关。使用免疫荧光和血凝抑制技术在表位水平证明了 CHIK 和 O'nyong nyong (ONN) 病毒之间的单向抗原关系。针对 ONN 病毒的 mAb 测试结果表明，虽然 ONN 病毒保留了大部分 CHIK 抗原位点，但许多 ONN 表位已经发生了足够的构象变化，使得针对它们制备的 mAb 无法识别 CHIK 病毒上的位点。
Chikungunya (CHIK) strains from Africa and Asia were shown to be antigenically closely related using a panel of monoclonal antibodies (mAb) prepared against strains H817 from Africa and PhH15483 from Asia. The one-way antigenic relationship between CHIK and o'nyong nyong (ONN) viruses was demonstrated at the epitope level using the immunofluorescence and haemagglutination inhibition techniques. Results of tests with mAb against ONN virus suggest that, while ONN virus has retained most of the CHIK antigenic sites, many of the ONN epitopes have undergone sufficient conformational change such that mAbs prepared against them do not recognize sites on CHIK virus.