Chemokine signaling regulates sensory cell migration in zebrafish
Chemokine signaling regulates sensory cell migration in zebrafish
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DOI：
10.1016/j.ydbio.2004.01.020
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发表时间：
2004-05-01
影响因子：
2.7
通讯作者：
Kuwada, JY
中科院分区：
文献类型：
--
作者：
Li, Q;Shirabe, K;Kuwada, JY
趋化因子在发育过程中多种细胞的迁移中发挥重要作用。最近的研究已经开始阐明趋化因子信号在神经系统发育中的重要性。为了更好地了解体内趋化因子的神经功能，我们在斑马鱼中分析了 SDF-1 通过其 CXCR4 受体的信号传导作用。 SDF-1-CXCR4 表达模式表明 SDF-(])-CXCR4 信号传导对于引导感觉细胞（称为后侧线迁移原基）的迁移很重要。转基因胚胎中配体的普遍诱导、配体或受体的反义敲除以及遗传受体突变都破坏了原基的迁移。此外，在内源性 SDF-1 被敲低的胚胎中，原基向外源性 SDF-1 迁移。这些数据表明，通过 CXCR4 介导的 SDF-1 信号传导可作为迁移原基的趋化剂，并且趋化因子信号传导对于指导原基迁移来说既是必要的也是充分的。 (C) 2004 Elsevier Inc. 保留所有权利。
Chemokines play an important role in the migration of a variety of cells during development. Recent investigations have begun to elucidate the importance of chemokine signaling within the developing nervous system. To better appreciate the neural function of chemokines in vivo, the role of signaling by SDF-1 through its CXCR4 receptor was analyzed in zebrafish. The SDF-1-CXCR4 expression pattern suggested that SDF-(])-CXCR4 signaling was important for guiding migration by sensory cells known as the migrating primordium of the posterior lateral line. Ubiquitous induction of the ligand in transgenic embryos, antisense knockdown of the ligand or receptor, and a genetic receptor mutation all disrupted migration by the primordium. Furthermore, in embryos in which endogenous SDF-1 was knocked down, the primordium migrated towards exogenous sources of SDF-1. These data demonstrate that SDF-1 signaling mediated via CXCR4 functions as chemoattractant for the migrating primordium and that chemokine signaling is both necessary and sufficient for directing primordium migration. (C) 2004 Elsevier Inc. All rights reserved.