Polycomb-mediated gene silencing by the BAH-EMF1 complex in plants
Polycomb-mediated gene silencing by the BAH-EMF1 complex in plants
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DOI：
10.1038/s41588-018-0190-0
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发表时间：
2018-09-01
期刊：
影响因子：
30.8
通讯作者：
He, Yuehui
中科院分区：
文献类型：
--
作者：
Li, Zicong;Fu, Xing;He, Yuehui
多梳蛋白在多细胞生物中实现全基因组转录抑制。进化上保守的Polycomb抑制复合物2（PRC 2）催化组蛋白H3 Lys 27三甲基化（H3 K27 me 3），其在动物中被Polycomb抑制复合物1（PRC 1）读取和影响，但该标记在植物中的解释仍不清楚。在这里，我们报告，在真双子叶植物拟南芥中的两个同源BAH（溴邻近同源）结构域的蛋白质形成一个植物特异性复合物与胚胎花1（EMF 1），BAH-EMF 1复合物（BAH-EMF 1c）读取和影响H3 K27 me 3标记和介导全基因组转录抑制。此外，在单子叶植物水稻中，拟南芥BAH结构域蛋白的同源物也结合甲基化的H3 K27，并与水稻EMF 1同源物形成复合物，表明BAH-EMF 1c在开花植物中是保守的。因此，我们的研究结果表明，植物特异性BAH-EMF 1c在高等植物中实现了PRC 1样功能，表明PRC 1活性在植物和动物中的趋同进化。
Polycomb proteins implement genome-wide transcriptional repression in multicellular organisms. The evolutionarily conserved Polycomb repressive complex 2 (PRC2) catalyzes histone H3 Lys27 trimethylation (H3K27me3) that is read and effected by Polycomb repressive complex 1 (PRC1) in animals, but the interpretation of this mark remains unclear in plants. Here we report that in the eudicot Arabidopsis thaliana two homologous BAH (Bromo adjacent homology) domain-containing proteins form a plant-specific complex with EMBRYONIC FLOWER 1 (EMF1), and that the BAH-EMF1 complex (BAH-EMF1c) reads and effects the H3K27me3 mark and mediates genome-wide transcriptional repression. Furthermore, in the monocot rice a homolog of the Arabidopsis BAH-domain proteins also binds methylated H3K27 and forms a complex with the rice homolog of EMF1, suggesting that BAH-EMF1c is conserved in flowering plants. Therefore, our results show that the plant-specific BAH-EMF1c fulfills PRC1-like functions in higher plants, suggesting a convergent evolution of PRC1 activity in plants and animals.