Effect of dual stimuli responsive dextran/nanocellulose polyelectrolyte complexes for chemophotothermal synergistic cancer therapy
Effect of dual stimuli responsive dextran/nanocellulose polyelectrolyte complexes for chemophotothermal synergistic cancer therapy
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DOI：
10.1016/j.ijbiomac.2019.05.218
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发表时间：
2019-08-15
影响因子：
8.2
通讯作者：
Thomas, Jefin P.
中科院分区：
文献类型：
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作者：
Anirudhan, T. S.;Sekhar, Chithra, V;Thomas, Jefin P.
双刺激响应纳米粒子已被开发用于结肠癌细胞的化学-光热协同治疗。这种新的体系是由胺化的纳米葡聚糖（AND）和羧化的纳米纤维素（CNC）沉积在化学修饰的氧化石墨烯（MGO）表面上通过层层（LbL）组装形成的。阳离子AND和阴离子CNC的交替层通过静电相互作用与MGO相互作用并形成MGO-AND/CNC纳米复合材料。利用π-π堆积和氢键相互作用，开发了MGO-AND/CNC用于抗癌药物姜黄素（CUR）的包封。检查了不同浓度的MGO和AND/CNC，发现颗粒的最佳流体动力学尺寸为158.0 nm，zeta电位为-45.9 +/-6.9 mV，包封效率为86.4 +/-4.7%。通过傅里叶变换红外光谱、扫描电子显微镜、透射电子显微镜、原子力显微镜、动态光散射和zeta电位测量对所得纳米复合材料进行了表征。药物释放试验表明，LbL MGO-AND/CNC在酸性环境中的释放速度比肠道pH值快得多。进行了细胞毒性试验，以证明载药MGO-AND/CNC响应近红外（NIR）激光发射而破坏HCT 116细胞的功效。研究结果表明，新型双敏感纳米颗粒允许细胞内姜黄素递送，并响应酸性环境或NIR激发。(C)2019爱思唯尔B.V.保留所有权利。
Dual stimuli responsive polyelectrolyte nanoparticles have been developed for chemo-photothermal synergistic therapy of colon cancer cells. This novel system is formed by layer by layer (LbL) assembly, which is composed of aminated nanodextran (AND) and carboxylated nanocellulose (CNC) deposited on the surface of chemically modified graphene oxide (MGO). The alternate layers of cationic AND and anionic CNC interact with MGO through electrostatic interaction and forms MGO-AND/CNC nanocomposite. The MGO-AND/CNC exploited for the encapsulation of anticancer drug curcumin (CUR) by pi-pi stacking and hydrogen bonding interactions. Various concentrations of MGO and AND/CNC were examined and the optimal hydrodynamic size of the particle was found to have 158.0 nm, zeta potential of -45.9 +/- 6.9 mV and encapsulation efficiency of 86.4 +/- 4.7%. The resulting nanocomposite was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering and zeta potential measurements. Drug release assay indicates that the LbL MGO-AND/CNC releases much faster in an acidic environment than intestinal pH. A cytotoxicity assay was conducted to prove the efficacy of drug loaded MGO-AND/CNC to destroy HCT116 cells in response to near-infrared (NIR) laser emission. Study results suggest the novel dual-sensitive nanoparticles allow intracellular curcumin delivery and respond to either acidic environments or NIR excitation. (C) 2019 Elsevier B.V. All rights reserved.