A high performance open-source syringe extruder optimized for extrusion and retraction during FRESH 3D bioprinting.
A high performance open-source syringe extruder optimized for extrusion and retraction during FRESH 3D bioprinting.
复制标题
高性能开源注射器挤出机，针对 FRESH 3D 生物打印过程中的挤出和缩回进行了优化。
DOI：
10.1016/j.ohx.2020.e00170
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发表时间：
2021
期刊：
影响因子：
2.2
通讯作者：
Feinberg,AdamW
中科院分区：
文献类型：
--
作者：
Tashman,JoshuaW;Shiwarski,DanielJ;Feinberg,AdamW
嵌入式3D生物打印的最新进展已将单个细丝的分辨率显著提高到100 µm以下;然而，使用如此小的细丝打印需要精确挤出纳升体积的生物墨水。可商购的生物打印机和挤出机是昂贵的，并且大多数利用气动控制，这限制了最小挤出体积并防止回缩（将生物墨水拉回到储存器中），这对于打印高分辨率特征和复杂的内部几何形状是必不可少的。在这里，我们展示了新一代的开源注射泵，专为基于挤出的软材料3D生物打印而设计：<$struder 4。<$struder 4利用了3D塑料打印的几何形状可定制性和易用性，同时通过集成大规模生产的高精度线性运动部件来提高性能。同时，这种新的注射泵仍然足够紧凑和轻便，可以在3D生物打印机上用于多材料生物打印。为了便于多种使用情况，该推杆4与一系列注射器兼容，包括一次性BD和汉密尔顿气密注射器。此外，我们描述了其他注射器的夹具设计过程。我们通过打印具有包含3.35 nL的单个细丝和宽度低至300 µm的通畅通道的I型胶原构建体，证明了具有2.5 mL汉密尔顿气密注射器的<$struder 4的性能。使用较小体积的汉密尔顿气密注射器，可以进一步提高该性能。因此，dstruder 4提供了一种开源解决方案，以当前嵌入式生物打印平台的分辨率极限打印软材料。
Recent advances in embedded 3D bioprinting have significantly improved the resolution of individual filaments to below 100 µm; however, printing with such small filaments requires accurate extrusion of nanoliter volumes of bioink. Commercially available bioprinters and extruders are expensive and most utilize pneumatic control, which limits the minimum extrusion volume and prevents retraction (pulling bioink back into the reservoir), which is essential to printing high resolution features and complex internal geometry. Here we present a new generation of our open-source syringe pump designed for extrusion-based 3D bioprinting of soft materials: the Replistruder 4. The Replistruder 4 takes advantage of the geometry customizability and ease of 3D plastic printing while improving performance by integrating mass produced high-precision linear motion components. Simultaneously this new syringe pump remains compact and lightweight enough for several to be utilized on a 3D bioprinter for multimaterial bioprinting. To facilitate multiple use cases the Replistruder 4 is compatible with a range of syringes including disposable BD and Hamilton gastight syringes. In addition, we describe the process of designing clamps for other syringes. We demonstrate the performance of a Replistruder 4 with a 2.5 mL Hamilton gastight syringe by printing collagen type I constructs with individual filaments comprising 3.35 nL and patent channels down to 300 µm in width. With smaller volume Hamilton gastight syringes this performance can be further improved. Thus, the Replistruder 4 provides an open-source solution to print soft materials at the resolution limits of current embedded bioprinting platforms.