Isolation and characterization of collagen A and B chains from chick embryos
Isolation and characterization of collagen A and B chains from chick embryos
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鸡胚胶原蛋白 A 和 B 链的分离和表征
DOI：
10.1016/0014-5793(79)80258-6
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发表时间：
1979
期刊：
影响因子：
3.5
通讯作者：
K. von der Mark
中科院分区：
文献类型：
--
作者：
H. von der Mark;K. von der Mark
从人胎膜中分离出两条新的胶原蛋白链，称为A链和B链，它们与I、II、III和IV型胶原蛋白的A链在遗传上不同[I]。这些胶原蛋白链也存在于人类皮肤和血管壁[2]、骨骼肌[3]以及骨和软骨[4]中；体外培养的平滑肌细胞可产生A链和B链[51]。从大多数组织和细胞培养中获得的B链是A链的两倍，这与存在一种亚基组成AB2（1.6）的胶原分子（~ rl (I)）~ ct2一致。然而，另一项研究表明，A链和B链是两种不同的三螺旋分子A3和Ba[4]的亚基。A， b -胶原与基底膜胶原在氨基酸组成上的相似性[1,4,6]及其在肌内膜中的定位表明，A， b -胶原可能是肌基板的一个组成部分。为了回答这个问题，我们尝试用鸡A、b胶原蛋白的特异性抗体在鸡肌肉细胞培养物中进行免疫组织学定位。最近的两项体外研究表明，在非哺乳动物物种中存在A、b胶原样分子。在新鲜分离的鸡肌腱成纤维细胞[8]和胚胎鸡神经视网膜细胞[9]的悬浮培养中，观察到两条链在与人的A链和B链相对应的位置上迁移。然而，并没有确凿的数据表明胶原蛋白是否
From human fetal membranes two new collagen chains, called A and B chains, have been isolated which are genetically different to the a-chains of types I, II, III and IV collagen [I]. These collagen chains were also found in human skin and in vessel walls [2], in skeletal bovine muscle [3] and in bone and cartilage [4]; in vitro A and B chains are produced by cultures of smooth muscle cells [51. From most tissues and cell cultures twice as much B chains as A chains were obtained which is consistent with the existence of a collagen molecule of the subunit composition AB2 (1.6) similar to type I collagen (~ rl (I))~ ct2. However, another study suggests that A and B chains are subunits of two different triple-helical molecules A3 and Ba [4]. The similarity of A, B-collagen to basement membrane collagen in terms of amino acid composition [1, 4, 6] and its localization in the endomysium [3] suggest the possibility that A, B-collagen may be a constituent of the muscle basal lamina. To answer this question, we attempted to localize this collagen type in chick muscle cell cultures immunohistologically using specific antibodies to chick A, B-collagen. The existence of A, B-collagen-like molecules in non-mammalian species was suggested recently by two in vitro studies.In suspension cultures of freshly-isolated chick tendon fibroblasts [8] and in cultures of embryonic chick neural retina cells [9] two chains were observed which migrated in the positions corresponding to human A and B chains. However, no conclusive data were available as to whether a collagen which is