Histochemical Localization of Acid Phosphatase in the Injured Rabbit Lens
Histochemical Localization of Acid Phosphatase in the Injured Rabbit Lens
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受伤兔晶状体中酸性磷酸酶的组织化学定位
DOI：
10.1159/000264747
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发表时间：
1975
影响因子：
2.1
通讯作者：
C. Harding
中科院分区：
文献类型：
--
作者：
N. Unakar;L. Binder;J. Reddan;C. Harding
用电镜组织化学方法研究了颗粒状白细胞，特别是中性粒细胞在兔晶状体损伤愈合过程中的作用和酸性磷酸酶的分布。颗粒状白细胞在损伤后约12小时后首次出现在创面凝血中。它们含有对酸性磷酸酶有阳性反应的颗粒。在12和24 h时，发现酸性磷酸酶定位在细胞外，在上皮细胞的顶端区域最为明显。这种定位在伤口周围最强烈，随着距离损伤部位的距离而减弱。在损伤后48小时，这种细胞外定位减少。讨论了水解酶在组织修复中的可能作用。
The role of granular leucocytes, particularly neutrophils, during wound healing in the injured rabbit lens and the distribution of acid phosphatase were investigated using electron microscopic histochemistry. Granular leucocytes first appeared in the wound coagulum approximately after 12 h following injury. They contained granules which gave a positive reaction for acid phosphatase. At 12 and 24 h acid phosphatase localization was found to be extracellular and was most pronounced at the apical region of the epithelial cells. This localization was most intense at the immediate periphery of the wound and decreased with distance from the site of injury. At 48 h following injury this extracellular localization decreased. The possible role of hydrolytic enzymes in tissue repair is discussed.