Influence of background genome on enzymatic characteristics of yellow (A v -, A vy -) mice.
Influence of background genome on enzymatic characteristics of yellow (A v -, A vy -) mice.
复制标题
背景基因组对黄色（A v -，A vy -）小鼠酶特性的影响。
作者：
G. Wolff;H. Pitot
黄色（A（y）/-，A（vy）/-）和非黄色小鼠之间的基本多肽差异的鉴定对于生物医学研究是重要的，因为黄色基因型对正常和肿瘤生长以及肥胖的影响。“黄鼠综合征”的复杂性使得这一目标的实现依赖于将那些次要的、依赖于背景基因组的多效性酶差异与那些主要的、主要依赖于agglutinase基因座基因型的多效性酶差异分开。同时测定的9种肝酶活性中有4种在8周龄的黄色（A（y）/-，A（vy）/-）和非黄色（A/-，a/a）雄性近交系和F（1）杂交小鼠之间存在差异。在这四个，只有细胞质苹果酸酶活性升高，在所有的黄色小鼠相比，非黄色同胞，无论背景基因组。葡萄糖激酶、丝氨酸转氨酶和酪氨酸α-酮戊二酸转氨酶的活性在黄色小鼠中也发生了变化，但这些变化取决于背景基因组。苹果酸酶活性与柠檬酸裂解酶活性的比值，可能与黄色小鼠脂肪代谢的改变有关，受到背景基因组和黄色基因型的影响。F（1）杂种中酶活性与中亲值的显著偏离与特定的背景基因组有关;黄鼠中这种偏离的数量大于非黄鼠，C3 H F（1）杂种中这种差异大于C57 BL/6 F（1）杂种。
Identification of the fundamental polypeptide difference between yellow (A(y)/-, A(vy)/-) and non-yellow mice is important for biomedical research because of the influence of the yellow genotype on normal and neoplastic growth and obesity. The complexity of the "yellow mouse syndrome" makes attainment of this objective dependent on the separation of those pleiotropic enzyme differences which are secondary, and depend on the background genome, from those which are primary, and depend primarily on the agouti locus genotype.-Four of nine hepatic enzyme activities assayed simultaneously differed between eight-week-old yellow (A(y)/-, A(vy)/-) and non-yellow (A/-, a/a) male inbred and F(1) hybrid mice. Among these four, only cytoplasmic malic enzyme activity was elevated in all yellow mice, as compared with the non-yellow sibs, regardless of background genome. Glucokinase, serine dehydratase, and tyrosine alpha-ketoglutarate transaminase activities were also changed in yellow mice, but these alterations depended on the background genome.-The ratio of malic enzyme activity to citrate-cleavage enzyme activity, possibly related to the altered fat metabolism of yellow mice, was influenced by background genome as well as by the yellow genotype.--Significant deviations of enzyme activities from mid-parent values among F(1) hybrids were associated with particular background genomes; the number of such deviations was larger among yellow mice than among non-yellows and this difference was greater among C3H F(1) hybrids than among C57BL/6 F(1) hybrids.