Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B(1) Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium.
Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B(1) Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium.
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DOI：
10.1155/2009/842626
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发表时间：
2009
影响因子：
3.4
通讯作者：
Markaki P
中科院分区：
文献类型：
--
作者：
Meimaroglou DM;Galanopoulou D;Markaki P
黄曲霉毒素B1 （AFB1）是某些曲霉种类在农产品上产生的致癌代谢物。AFB1的生物合成受到茉莉酸及其甲基lester （MeJA）的影响，MeJA是一种从亚油酸中提取的植物生长调节剂。本研究报道了在酵母抽提物蔗糖（YES）培养基中添加三种不同浓度的MeJA对寄生蜂生长和AFB1产量的影响；即10−2m、10−4m和10−6m。免疫亲和法和高效液相色谱法测定AFB1。10−4和10−6 M浓度的MeJA对菌丝生长无显著影响，但在培养第7天后影响了AFB1的产生；第12天，AFB1产量比对照样品（分别添加10−6 M和10−4 M MeJA）分别提高了212.7%和141.6%。用10−2 M MeJA处理寄生蜂培养物也抑制了菌丝生长和AFB1的产生。这些结果表明，MeJA对寄生蜂AFB1合成的影响取决于MeJA的浓度。
Aflatoxin B1 (AFB1) is a carcinogenic metabolite produced by certain Aspergillus species on agricultural commodities. AFB1 biosynthesis is affected by jasmonic acid and also by its methylester (MeJA), a plant growth regulator derived from linoleic acid. This study reports the effect of MeJA on the growth of A. parasiticus and AFB1 output in yeast extract sucrose (YES) medium when added at three different concentrations; namely, 10−2 M, 10−4 M, and 10−6 M. AFB1 determination was performed by immunoaffinity and HPLC. MeJA at 10−4 and 10−6 M concentrations had no significant effect on mycelial growth but did affect AFB1 production after the 7th day of incubation; on the 12th day, AFB1 production was increased by 212.7% and 141.6% compared to the control samples (addition of 10−6 M and 10−4 M MeJA, resp.). Treatment of A. parasiticus cultures with 10−2 M MeJA inhibited mycelial growth and AFB1 production as well. These results suggest that the effect of MeJA on AFB1 biosynthesis by A. parasiticus depends on the MeJA concentration used.