AN IMPROVED PROCEDURE FOR BILE-ACID EXTRACTION AND PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT
AN IMPROVED PROCEDURE FOR BILE-ACID EXTRACTION AND PURIFICATION AND TISSUE DISTRIBUTION IN THE RAT
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DOI：
10.1007/bf02535238
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发表时间：
1989-03-01
期刊：
影响因子：
1.9
通讯作者：
GALLAHER, DD
中科院分区：
文献类型：
--
作者：
LOCKET, PL;GALLAHER, DD
使用内源性放射性标记的组织和粪便比较了两种胆汁酸提取程序。Sethanes等人的方法（J. Lipid Res.24，1085-1100，1983）导致基本完全提取，而Manes和Schneider的方法（J. Lipid Res.6，376-377，1971）给出56- 82%的回收率。Settal等人的方法所需的时间可以大大减少，而不会损失提取效率。使用内源性标记材料的提取物，开发了使用C18固相萃取柱的纯化程序，回收> 90%的胆汁酸。测定了胆汁酸在大鼠肠道和肝脏中的分布。
Two bile acid extraction procedures were compared using endogenously radiolabeled tissues and feces. The method of Setchell et al. (J. Lipid Res. 24, 1085-1100, 1983) resulted in essential complete extraction, whereas that of Manes and Schneider (J. Lipid Res. 6, 376-377, 1971) gave recoveries between 56-82%. The time requirement for the method of Setchell et al. could be drastically reduced with no loss in extraction efficiency. Using extracts from endogenously labeled material, a purification procedure using C18 solid-phase extraction cartridges was developed that recovers > 90% of bile acids. The distribution of bile acids within the intestinal tract and liver of the rat was determined.