Expression of mRNAs encoding for α and β integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts
Expression of mRNAs encoding for α and β integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts
复制标题
DOI：
10.1177/00220345000790091201
复制
发表时间：
2000-09-01
影响因子：
7.6
通讯作者：
Tenenbaum, H
中科院分区：
文献类型：
--
作者：
Bolcato-Bellemin, AL;Elkaim, R;Tenenbaum, H
牙齿运动的生物学机制源于结缔组织对外源性机械力的细胞反应。在这些反应中，发生了细胞外基质的降解，但对分子基础的鉴定以及与这种降解有关的成分知之甚少。为了进行这一鉴定，我们将从牙周韧带（pdl）和牙龈（HGFs）中获得的人成纤维细胞进行连续拉伸，以量化编码各种金属蛋白酶（MMPs）、它们的组织抑制剂（TIMPs）和整合素亚基的mrna。两种细胞系都通过诱导编码MMP-1、MMP-2、TIMP-1和TIMP-2的mrna表达而发生反应，而其他mrna则没有变化（MT1-MMP、TIMP-3）或不表达（MMP-9）。pdl选择性地表达编码α 4和α v的mrna，在拉伸下没有可测量的差异，编码α 6和β 1的mrna增加，编码α 5的mrna减少。hgf增加了编码α 2、α 6、β 1和β 3的mrna，减少了编码α 3的mrna。我们的数据分析表明，拉伸的HGFs和pdl诱导编码MMPs和TIMPs的mrna模式相同，但编码各种整合素亚基的mrna模式不同，这些整合素亚基在机械转导中扮演蛋白质受体的角色。
The biological mechanisms of tooth movement result from the cellular responses of connective tissues to exogenous mechanical forces. Among these responses, the degradation of the extracellular matrix takes place, but the identification of the molecular basis as well as the components implicated in this degradation are poorly understood. To contribute to this identification, we subjected human fibroblasts obtained from the periodontal ligament (PDLs) and from the gingiva (HGFs) to a continuous stretch to quantify the mRNAs encoding for various metalloproteinases (MMPs), their tissue inhibitors (TIMPs), and or and beta integrin subunits. Both cell lines reacted by inducing the expression of the mRNAs encoding for MMP-1, MMP-2, TIMP-1, and TIMP-2, while other mRNAs did not vary (MT1-MMP, TIMP-3) or were not expressed (MMP-9). PDLs expressed selectively the mRNAs encoding for alpha 4 and alpha v, with no difference measurable under stretching, while the mRNAs encoding for alpha 6 and beta 1 were increased and the one encoding for alpha 5 was decreased. HGFs increased the mRNAs encoding for alpha 2, alpha 6, beta 1, and beta 3 and decreased the one encoding for alpha 3. Analysis of our data indicated that stretched HGFs and PDLs induced the same pattern of mRNAs encoding for MMPs and TIMPs but differed for those encoding various integrin subunits, known to act as protein receptors in mechanotransduction.