In vivo visualization of chromosomes using lac operator-repressor binding
In vivo visualization of chromosomes using lac operator-repressor binding
复制标题
DOI：
10.1016/s0962-8924(97)01211-7
复制
发表时间：
1998-03-01
影响因子：
19
通讯作者：
Straight, AF
中科院分区：
文献类型：
--
作者：
Belmont, AS;Straight, AF
本文描述了一种基于 lac 阻遏物对 lac 算子直接修饰的识别的染色体动力学直接体内可视化新技术。该方法允许标记特定的染色体位点，从而以最小的结构扰动进行原位定位。使用 GFP 阻遏物融合蛋白或免疫荧光通过光学显微镜进行检测，可以通过使用免疫金染色的更高分辨率电子显微镜进行补充。该方法的应用将有助于研究间期染色体动力学，以及缺乏细胞学浓缩染色体的生物体细胞分裂过程中的染色体分离。
This article describes a new technique for direct, in vivo visualization of chromosome dynamics based on lac repressor recognition of direct repents of the lac operator. The method allows the tagging of specific chromosomal sites and thus in situ localization with minimal perturbation of structure. Detection by light microscopy, using GFP-repressor fusion proteins or immunofluorescence, can be complemented by higher-resolution electron microscopy using immunogold staining. Applications of this method will facilitate the investigation of interphase chromosome dynamics, as well as chromosome segregation during cell division in organisms that lack cytologically condensed chromosomes.