Predation of the Newly Invasive Pest Megacopta cribraria (Hemiptera: Plataspidae) in Soybean Habitats Adjacent to Cotton by a Complex of Predators
Predation of the Newly Invasive Pest Megacopta cribraria (Hemiptera: Plataspidae) in Soybean Habitats Adjacent to Cotton by a Complex of Predators
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DOI：
10.1603/ec13356
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发表时间：
2014-06-01
影响因子：
2.2
通讯作者：
Hu, J. S.
中科院分区：
文献类型：
--
作者：
Greenstone, M. H.;Tillman, P. G.;Hu, J. S.
葛虫（Megacopta cribraria (F.)）（半翅目：Plataspidae）是一种新入侵的外来昆虫，主要在美国东南部的葛上发现，但也在大豆上发现。我们使用分子肠道内容分析来记录大豆中昆虫和蜘蛛对这种害虫的捕食，并检测捕食者肠道中特定作物替代猎物的残留物，作为捕食者在大豆和邻近棉花之间迁移的标记。 M. cribraria 仅在大豆上发现。两种作物上的八种本土多面性捕食者通过特定 PCR 检测害虫 DNA 呈阳性：Geocoris punctipes (Say)、Geocoris uliginosus (Say)、Orius insidiosus (Say)、Podisus maculiventris (Say)、Hippodamia converens Guerin-Meneville、Zelus renardii (Kolenati)、Oxyopes salticus Hentz 和 Peucetia草绿色（亨茨）；第九种捕食者，外来红火蚁 (Solenopsis invicta Buren)，其 M. cribraria DNA 筛查结果也呈阳性。 P. viridans 是唯一一种仅在一种作物——棉花中检测出这种入侵性害虫 DNA 呈阳性的节肢动物。两种以植物为食的五叶目物种，Piezodorus guildinii (Westwood) 和Thyanta custator (F.)，仅在大豆上发现，而另一种，Euschistus tristigmus (Say)，在本研究中是棉花特有的。对棉花中的 M. cribraria、P. guildinii 和 T. custator 以及大豆中的 M. cribraria 和 E. tristigmus 组合的捕食情况的检测表明，这些捕食者分散在作物之间。这些结果强烈支持利用棉花附近的大豆栖息地作为针对 M. cribraria 的保护性生物控制策略的一部分。这是第一份通过分子肠道内容分析记录这种外来害虫在野外捕食的报告。
The kudzu bug, Megacopta cribraria (F.) (Hemiptera: Plataspidae), is a newly invasive exotic insect found primarily on kudzu, but also on soybean, in the southeastern United States. We used molecular gut-content analysis to document predation on this pest by insects and spiders in soybean, and to detect remains of crop-specific alternative prey in predators' guts as markers of predator migration between soybean and adjacent cotton. M. cribraria was found exclusively on soybean. Eight native generalist predators over both crops screened positive by specific PCR for DNA of the pest: Geocoris punctipes (Say), Geocoris uliginosus (Say), Orius insidiosus (Say), Podisus maculiventris (Say), Hippodamia convergens Guerin-Meneville, Zelus renardii (Kolenati), Oxyopes salticus Hentz, and Peucetia viridans (Hentz); a ninth predator, the exotic Solenopsis invicta Buren, also screened positive for M. cribraria DNA. P. viridans was the only arthropod that tested positive for DNA of this invasive pest in only one crop, cotton. Two plant-feeding pentatomid species, Piezodorus guildinii (Westwood) and Thyanta custator (F.), were found exclusively on soybean, and another, Euschistus tristigmus (Say), was specific to cotton in the context of this study. Detection of predation on a combination of M. cribraria and P. guildinii and T. custator in cotton and M. cribraria and E. tristigmus in soybean demonstrated that these predators dispersed between crops. These results strongly support the use of soybean habitats adjacent to cotton as part of a conservation biological control strategy against M. cribraria. This is the first report documenting predation on this exotic pest in the field via molecular gut-content analysis.