A Translationally Controlled Tumor Protein Negatively Regulates the Hypersensitive Response in Nicotiana benthamiana
A Translationally Controlled Tumor Protein Negatively Regulates the Hypersensitive Response in Nicotiana benthamiana
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DOI：
10.1093/pcp/pct090
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发表时间：
2013-08-01
影响因子：
4.9
通讯作者：
Kiba, Akinori
中科院分区：
文献类型：
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作者：
Gupta, Meenu;Yoshioka, Hirofumi;Kiba, Akinori
我们一直在烟草植物中分离和鉴定青枯雷尔氏菌响应基因（RsRGs）。在这项研究中，我们专注于RsRG 308，我们将其重新命名为NbTCTP（N。本塞姆氏菌抑制性控制的肿瘤蛋白），因为它编码与抑制性控制的肿瘤蛋白相似的多肽。在NbTCTP沉默的N.本萨米亚那（benthamiana）植物受到R.青枯菌8107（Rs 8107）。Rs 8107群体显著减少，而hin 1基因表达在沉默植物中增强。在用菊苣假单胞菌和P.你好NbTCTP的沉默也加速了由农杆菌介导的HR诱导剂（如AvrA、BAX、INF 1和NbMEK 2（DD））的瞬时表达诱导的HR细胞死亡。NbTCTP沉默增强NbrbohB和NbMEK 2介导的活性氧产生，导致HR。NbTCTP全长序列和Bcl-xL结构域的瞬时表达降低了由农杆菌介导的HR诱导剂瞬时表达诱导的HR细胞死亡。NbTCTP沉默的植物也表现出轻微的矮化表型。因此，NbTCTP可能在HR期间的细胞死亡调节中发挥作用，以微调程序性细胞死亡相关的植物防御反应。
We have been isolating and characterizing Ralstonia solanacearum-responsive genes (RsRGs) in Nicotiana plants. In this study we focused on RsRG308, which we renamed NbTCTP (N. benthamiana translationally controlled tumor protein) because it encodes a polypeptide showing similarity to translationally controlled tumor proteins. Induction of the hypersensitive response (HR) was accelerated in NbTCTP-silenced N. benthamiana plants challenged with R. solanacearum 8107 (Rs8107). The Rs8107 population decreased significantly, whereas hin1 gene expression was enhanced in the silenced plant. Accelerated induction of HR was observed in NbTCTP-silenced plants inoculated with Pseudomonas cichorii and P. syringae pv. syringae. Silencing of NbTCTP also accelerated the induction of HR cell death by Agrobacterium-mediated transient expression of HR inducers, such as AvrA, BAX, INF1 and NbMEK2(DD). NbTCTP silencing enhanced NbrbohB- and NbMEK2-mediated reactive oxygen species production, leading to HR. Transient expression of both the full-length sequence and the Bcl-xL domain of NbTCTP decreased HR cell death induced by Agrobacterium-mediated transient expression of HR inducers. NbTCTP-silenced plants also showed slightly dwarf phenotypes. Therefore, NbTCTP might have a role in cell death regulation during HR to fine-tune programmed cell death-associated plant defense responses.