Cortactin is required for integrin-mediated cell spreading
Cortactin is required for integrin-mediated cell spreading
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DOI：
10.1016/j.imlet.2005.11.011
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发表时间：
2006-04-15
影响因子：
4.4
通讯作者：
Buday, L
中科院分区：
文献类型：
--
作者：
Illés, A;Enyedi, B;Buday, L
Cortactin是一种含有SH3结构域的蛋白质，有助于形成动态的皮质肌动蛋白相关结构，如片状脂膜和膜褶皱。在这里，我们表明，无论是GFP标记的N-末端还是C-精液半部分的表达都显著地抑制了COS7细胞在纤维连接蛋白上的扩散。将失活点突变引入Cortactin的C-末端半部分的SH3结构域，可以暂停该构建体的显性负面影响。此外，基于载体的RNA干扰被用来下调细胞内源性皮质素的水平。我们证明了皮质素缺陷细胞不能扩散。这些结果表明，皮质素是整合素介导的信号通路所必需的。(C)2005爱思唯尔B.V.保留所有权利。
Cortactin is an SH3 domain-containing protein that contributes to the formation of dynamic cortical actin-associated structures, such as lamellipodia and membrane ruffles. Here we show that expression of either the GFP-tagged N-terminal or the C-seminal halves of cortactin inhibits significantly the spreading of COS7 cells on fibronectin. Introducing inactivating point mutation into the SH3 domain of the C-terminal half of cortactin suspends the dominant negative effect of the construct. In addition, a vector-based RNA interference was used to knock-down endogenous level of cortactin in cells. We demonstrate that cortactin deficient cells were not able to spread. These results suggest that cortactin is required for integrin-mediated signalling pathways. (C) 2005 Elsevier B.V All rights reserved.