CeMbio-TheCaenorhabditis elegansMicrobiome Resource
CeMbio-TheCaenorhabditis elegansMicrobiome Resource
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DOI：
10.1534/g3.120.401309
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发表时间：
2020-09-01
影响因子：
2.6
通讯作者：
Samuel, Buck S.
中科院分区：
文献类型：
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作者：
Dirksen, Philipp;Assie, Adrien;Samuel, Buck S.
通过测序对微生物群落的研究揭示了微生物群落组成与相应寄主物种的各种生活史特征之间的过度相关性。然而，即使是在简单的生物体中，从相关性推断因果关系通常也会受到微生物组分的绝对复杂性的阻碍。合成群落提供了一种有效的方法来推断宿主-微生物组系统中的因果关系。然而，现有的群落存在几个缺点，如人工(因此非自然)选择微生物、微生物与宿主不匹配(例如，灵生小鼠体内的人类微生物)，或者宿主缺乏遗传可控性。在这里，我们介绍CeMbio，一个简化的自然线虫微生物群，源于我们之前对这种线虫的自然微生物组的荟萃分析。CeMbio资源服从于C的所有优势。线虫模型系统，包括菌株很容易培养，它们都单独在蠕虫肠道定居，并组成一个强大的群落，明显影响线虫的生活史。此外，还为CeMbio菌株开发了几种工具，包括诊断PCR引物、完全测序的基因组和代谢网络模型。有了CeMbio，我们提供了一个多功能的资源和工具箱，用于深入剖析自然相关的宿主-微生物组相互作用公司。优雅女装。
The study of microbiomes by sequencing has revealed a plethora of correlations between microbial community composition and various life-history characteristics of the corresponding host species. However, inferring causation from correlation is often hampered by the sheer compositional complexity of microbiomes, even in simple organisms. Synthetic communities offer an effective approach to infer cause-effect relationships in host-microbiome systems. Yet the available communities suffer from several drawbacks, such as artificial (thus non-natural) choice of microbes, microbe-host mismatch (e.g., human microbes in gnotobiotic mice), or hosts lacking genetic tractability. Here we introduce CeMbio, a simplified naturalCaenorhabditis elegansmicrobiota derived from our previous meta-analysis of the natural microbiome of this nematode. The CeMbio resource is amenable to all strengths of theC. elegansmodel system, strains included are readily culturable, they all colonize the worm gut individually, and comprise a robust community that distinctly affects nematode life-history. Several tools have additionally been developed for the CeMbio strains, including diagnostic PCR primers, completely sequenced genomes, and metabolic network models. With CeMbio, we provide a versatile resource and toolbox for the in-depth dissection of naturally relevant host-microbiome interactions inC. elegans.