Biomechanical assessment of myocardial infarction using optical coherence elastography.
Biomechanical assessment of myocardial infarction using optical coherence elastography.
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DOI：
10.1364/boe.9.000728
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发表时间：
2018-02
影响因子：
3.4
通讯作者：
Shang Wang;Manmohan Singh;Thuy Tien Tran;John P. Leach;S. Aglyamov;I. Larina;James F. Martin;K. Larin
中科院分区：
文献类型：
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作者：
Shang Wang;Manmohan Singh;Thuy Tien Tran;John P. Leach;S. Aglyamov;I. Larina;James F. Martin;K. Larin
心肌梗死（MI）导致心肌细胞损失、心脏功能受损和心力衰竭。对缺血性心脏病小鼠模型心肌的分子遗传学分析为心肌再生机制提供了新的思路，为心肌梗死后的治疗提供了新的思路。虽然生物力学因素被认为是心肌细胞增殖的一个重要方面，但在小鼠MI模型中对心脏进行机械评估的方法有限。这阻碍了进一步理解组织生物力学在心脏再生中的作用。在这里，我们报告光学相干弹性成像（OCE）的小鼠心脏后MI。进行左冠状动脉前降支的手术结扎以诱导心脏梗死。两个OCE方法与方向相关的弹性波传播和空间分辨位移阻尼的评估提供了补充分析的左心室。与假手术组相比，心梗后第6周，心梗心脏的特征是纤维化瘢痕区域弹性波速度降低，固有频率降低，组织水平的机械各向异性降低，表明刚度降低且各向同性增加。我们的研究结果表明，OCE可以用于非破坏性的生物力学表征的MI在小鼠模型，这可能是一个有用的工具，在心脏修复的研究。
Myocardial infarction (MI) leads to cardiomyocyte loss, impaired cardiac function, and heart failure. Molecular genetic analyses of myocardium in mouse models of ischemic heart disease have provided great insight into the mechanisms of heart regeneration, which is promising for novel therapies after MI. Although biomechanical factors are considered an important aspect in cardiomyocyte proliferation, there are limited methods for mechanical assessment of the heart in the mouse MI model. This prevents further understanding the role of tissue biomechanics in cardiac regeneration. Here we report optical coherence elastography (OCE) of the mouse heart after MI. Surgical ligation of the left anterior descending coronary artery was performed to induce an infarction in the heart. Two OCE methods with assessment of the direction-dependent elastic wave propagation and the spatially resolved displacement damping provide complementary analyses of the left ventricle. In comparison with sham, the infarcted heart features a fibrotic scar region with reduced elastic wave velocity, decreased natural frequency, and less mechanical anisotropy at the tissue level at the sixth week post-MI, suggesting lower and more isotropic stiffness. Our results indicate that OCE can be utilized for nondestructive biomechanical characterization of MI in the mouse model, which could serve as a useful tool in the study of heart repair.