Tumor necrosis factor-α converting enzyme (TACE) increases RANKL expression in osteoblasts and serves as a potential biomarker of periodontitis
Tumor necrosis factor-α converting enzyme (TACE) increases RANKL expression in osteoblasts and serves as a potential biomarker of periodontitis
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DOI：
10.5483/bmbrep.2011.44.7.473
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发表时间：
2011-07-31
期刊：
影响因子：
3.8
通讯作者：
Cho, Je-Yoel
中科院分区：
文献类型：
--
作者：
Lee, Ji-Hyun;Choi, Young-Jin;Cho, Je-Yoel
牙周炎是一种非常普遍的疾病。因此，迫切需要牙周炎的早期和标准诊断的生物标志物。TACE是一种膜结合金属蛋白酶。虽然最近的一项研究表明，在牙周病期间，GCF中的TACE水平升高，但在慢性牙周炎的不同阶段，GCF中的TACE水平尚未确定。在这里，我们分析了牙周病受试者GCF中TACE的蛋白水平，并证实中度牙周炎组中TACE的蛋白水平较高。已知TACE是刺激成骨细胞中RANKL分泌的NF-κ B配体。为了了解TACE对成骨细胞中RANKL和OPG的影响，我们用TACE处理MG 63细胞。我们观察到RANKL蛋白表达增加，但OPG蛋白表达减少。我们的数据表明，TACE可以诱导RANKL表达，促进破骨细胞生成，从而恶化牙周炎的结果。[BMB报告2011; 44（7）：473-477]
Periodontitis is a very prevalent disease. Therefore, biomarkers for the early and standard diagnoses of periodontitis are urgently needed. TACE is a membrane-bound metalloprotease. Although a recent study suggested that TACE levels in GCF are elevated during periodontal disease, the levels of TACE in GCF at different stages of chronic periodontitis have not been determined. Here, we analyzed the protein levels of TACE in GCF from periodontal disease subjects and confirmed that the protein levels of TACE were higher in the moderate periodontitis groups. TACE is known to be a NF-kappa B ligand that stimulates RANKL secretion in osteoblasts. To understand the effects of TACE on RANKL and OPG in osteoblasts, we treated MG63 cells with TACE. We observed an increase in RANKL protein expression but a decrease in OPG protein expression. Our data suggest that TACE can induce RANKL expression and promote osteoclastogenesis, thus worsening the outcome of periodontitis. [BMB reports 2011; 44(7): 473-477]