Redesigning flow injection after 40 years of development: Flow programming
Redesigning flow injection after 40 years of development: Flow programming
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DOI：
10.1016/j.talanta.2017.08.061
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发表时间：
2018-01-01
期刊：
影响因子：
6.1
通讯作者：
Ruzicka, Jaromir (Jarda)
中科院分区：
文献类型：
--
作者：
Ruzicka, Jaromir (Jarda)
通过流动注射 (FI) 实现的基于试剂的测定的自动化以样品处理为基础，其中样品连续流向并通过检测器以对目标分析物进行定量。这种方法的致命弱点是自动分析仪的遗留问题，即不断消耗试剂并不断产生化学废物。然而，在精确泵送最新进展的辅助下，结合阀门实验室技术，流量编程允许围绕单个汇合点设计 FI 歧管，通过一系列反向流动，样品和试剂通过该汇合点顺序引导。这种方法的结果是类似于传统 FI 的样品/试剂混合，减少了样品和试剂消耗，并使用停流技术来提高化学反应的产率。通过磷酸盐、硝酸盐、亚硝酸盐和葡萄糖的常用分光光度测定示例记录了可编程流动注射 (01) 的可行性。实验详细信息和其他信息可在在线教程 http://www.flowinjectiontutorial.com/ 中找到
Automation of reagent based assays, by means of Flow Injection (FI), is based on sample processing, in which a sample flows continuously towards and through a detector for quantification of the target analyte. The Achilles heel of this methodology, the legacy of Auto Analyzer, is continuous reagent consumption, and continuous generation of chemical waste. However, flow programming, assisted by recent advances in precise pumping, combined with the lab-on-valve technique, allows the FI manifold to be designed around a single confluence point through which sample and reagents are sequentially directed by means of a series of flow reversals. This approach results in sample/reagent mixing analogous to the traditional FI, reduces sample and reagent consumption, and uses the stop flow technique for enhancement of the yield of chemical reactions. The feasibility of programmable Flow Injection (01) is documented by example of commonly used spectrophotometric assays of, phosphate, nitrate, nitrite and glucose. Experimental details and additional information are available in online tutorial http://www.flowinjectiontutorial.com/