Concentration, distribution and expression of interleukin-5 in human nasal polyp tissues.
Concentration, distribution and expression of interleukin-5 in human nasal polyp tissues.
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DOI：
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发表时间：
2002-09
影响因子：
6.1
通讯作者：
R. Xu;Yuan Li;M. Xie;Geng Xu;Gehua Zhang;Shu-fang Wang
中科院分区：
文献类型：
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作者：
R. Xu;Yuan Li;M. Xie;Geng Xu;Gehua Zhang;Shu-fang Wang
目的研究IL-5在鼻息肉组织中的浓度、分布和表达，探讨其在嗜酸性粒细胞聚集微环境分化中的意义。方法采用酶联免疫吸附试验（ELISA）和免疫组化法检测40例鼻息肉患者鼻息肉组织中IL-5的含量和表达，并以鼻息肉患者和健康志愿者下鼻甲黏膜为对照。结果鼻息肉组织中IL-5浓度显著高于鼻甲骨粘膜组织（P0.05）.变应性鼻炎患者鼻息肉组织中IL-5浓度显著高于非变应性鼻炎患者（P0.05）。80.1%的嗜酸性粒细胞表达IL-5，90.9%的IL-5阳性细胞为嗜酸性粒细胞。只有3.7%的淋巴细胞和中性粒细胞IL-5阳性; IL-5在上皮细胞中未检测到。鼻息肉组织中IL-5的表达明显强于鼻甲黏膜组织（P0.05）。变应性鼻炎患者鼻息肉组织中嗜酸性粒细胞IL-5的表达明显高于非变应性鼻炎患者（P0.05）。结论IL-5是鼻息肉组织嗜酸性粒细胞浸润的重要细胞因子。
OBJECTIVES To study the concentration, distribution and expression of IL-5 in nasal polyp tissues and explore its significance in micro-environment differentiation of eosinophil accumulation. METHODS The concentration and expression of IL-5 in nasal polyp tissues of 40 patients were determined by ELISA and immunohistochemistry and inferior turbinate mucosa from patients with nasal polyps and healthy volunteers were used as control. RESULTS IL-5 concentration in polyp tissues was significantly higher than that in turbinate mucosa (P 0.05). IL-5 concentrations in polyp tissues were markedly higher in patients with allergic rhinitis compared with those without (P 0.05). 80.1% of the eosinophils were positive for IL-5 and 90.9% of IL-5 positive cells were eosinophils. Only 3.7% of lymphocytes and neutrophils were positive for IL-5; IL-5 was not detectable in epithelial cells. IL-5 expression in eosinophils of polyp tissues was remarkably stronger than that of the turbinate mucosa (P 0.05). IL-5 expression of eosinophils in polyp tissue was significantly stronger in patients with allergic rhinitis compared with those without (P 0.05). CONCLUSION IL-5 is the key cytokine in eosinophilic pathologic mechanisms in nasal polyp tissues.