Synthesis, DNA-binding and photocleavage studies of cobalt(III) mixed-polypyridyl complexes: [Co(phen)(2)(dpta)](3+) and [Co(phen)(2)(amtp)](3+).
Synthesis, DNA-binding and photocleavage studies of cobalt(III) mixed-polypyridyl complexes: [Co(phen)(2)(dpta)](3+) and [Co(phen)(2)(amtp)](3+).
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DOI：
10.1016/j.jinorgbio.2006.11.008
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发表时间：
2007-03
影响因子：
3.9
通讯作者：
B. Peng;Hui Chao;Bin Sun;Hong Li;Feng Gao;L. Ji
中科院分区：
文献类型：
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作者：
B. Peng;Hui Chao;Bin Sun;Hong Li;Feng Gao;L. Ji
合成并表征了两种新型混配多吡啶钴配合物[Co（phen）2（dpta）]3+和[Co（phen）2（amtp）]3+（phen= 1，10-菲咯啉，dpta=联吡啶并[3，2-a;2′，3 ′-c]噻吩并[3，4-c]吖嗪，amtp=3-氨基-1，2，4-三嗪并[5，6-f] 1，10-菲咯啉）.通过光谱法、循环伏安法和粘度法研究了这些配合物与小牛胸腺DNA的相互作用。结果表明，这两种配合物通过嵌入模式与DNA结合。此外，已发现这些Co（III）配合物在365 nm照射下促进质粒DNA pBR 322的光切割。机理研究表明，羟基自由基（OH）可能是[Co（phen）2（dpta）]3+切割质粒DNA的反应活性物种，超氧阴离子自由基（O2-）在[Co（phen）2（amtp）]3+切割质粒DNA的反应中起关键作用。
Two novel cobalt(III) mixed-polypyridyl complexes [Co(phen)2(dpta)]3+and [Co(phen)2(amtp)]3+(phen=1,10-phenanthroline, dpta=dipyrido-[3,2-a;2′,3′-c]- thien-[3,4-c]azine, amtp=3-amino-1,2,4-triazino[5,6-f]1,10-phenanthroline) have been synthesized and characterized. The interaction of these complexes with calf thymus DNA was investigated by spectroscopic, cyclic voltammetry, and viscosity measurements. Results suggest that the two complexes bind to DNA via an intercalative mode. Moreover, these Co(III) complexes have been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365nm. The mechanism studies reveal that hydroxyl radical (OH) is likely to be the reactive species responsible for the cleavage of plasmid DNA by [Co(phen)2(dpta)]3+and superoxide anion radical (O2-) acts as the key role in the cleavage reaction of plasmid DNA by [Co(phen)2(amtp)]3+.