Enhanced rates of fluid pinocytosis during exponential growth and monolayer regeneration by cultured arterial endothelial cells
Enhanced rates of fluid pinocytosis during exponential growth and monolayer regeneration by cultured arterial endothelial cells
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培养的动脉内皮细胞指数生长和单层再生期间液体胞饮作用速率增强
DOI：
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发表时间：
1980
影响因子：
5.6
通讯作者：
S. Schwartz
中科院分区：
文献类型：
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作者：
P. Davies;Sydney C. Selden;S. Schwartz
牛主动脉内皮细胞在体外生长状态的各种操作过程中，测量流体胞饮率。稀疏接种的培养物呈指数生长，直至形成汇合单层，此时生长减慢。生长速率的这种变化与胞饮速率下降到生长培养物中的约三分之一相一致。在随后达到最大细胞密度的融合单层，胞饮率保持恒定。通过放射自显影术测量的3 H-胸苷标记指数与胞饮率之间存在密切相关性。融合单层的机械“创伤”导致细胞迁移和增殖。“受伤”后24小时，每毫克的胞饮率。细胞蛋白质含量显著增加。当单层细胞的再生被细胞松弛素B阻断时，胞饮作用保持与未损伤的融合单层细胞相同的速率。这些实验支持并延伸到内皮细胞，早期的观察结果表明，生长细胞中的胞饮作用比非生长的静止细胞中的速率更高。此外，他们提出了这样一种可能性，即在体内通过受体依赖性流体胞饮作用跨内皮转运大分子（如脂蛋白）可能会被内皮的生长状态改变。
Rates of fluid pinocytosis by bovine aortic endothelial cells were measured during various manipulations of growth status in vitro. Sparsely seeded cultures grew exponentially until a confluent monolayer was formed, at which time growth slowed. This change in growth rate coincided with a decline in the rate of pinocytosis to about one‐third that in the growing cultures. During the subsequent attainment of maximal cell density in the confluent monolayer, the pinocytic rate remained constant. There was close correlation between 3H‐thymidine labelling indices, as measured by autoradiography, and the rates of pinocytosis. Mechanical “wounding” of the confluent monolayer resulted in cell migration and proliferation. Twenty‐four hours after “wounding,” rates of pinocytosis per mg. cell protein were significantly enhanced. When regeneration of the monolayer was blocked by cytochalasin B, pinocytosis remained at the same rate as in the uninjured, confluent monolayer. These experiments support, and extend to endothelium, earlier observations that in growing cells pinocytosis proceeds at a higher rate than in non‐growing, quiescent cells. Furthermore, they raise the possibility that the transendothelial transport of macromolecules such as lipoproteins by receptor‐in‐dependent fluid pinocytosis in vivo may be altered by the growth status of the endothelium.