Screening and genetic analysis of ethylene-response mutants in etiolated rice seedlings
Screening and genetic analysis of ethylene-response mutants in etiolated rice seedlings
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黄化水稻幼苗乙烯反应突变体的筛选及遗传分析
DOI：
10.21769/bioprotoc.3001
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发表时间：
2018
期刊：
影响因子：
0.8
通讯作者：
Jin-Song Zhang
中科院分区：
文献类型：
--
作者：
Cui-Cui Yin;Biao Ma;He Zhao;Shou-Yi Chen;Jin-Song Zhang
[摘要] 乙烯是最简单的气态植物激素，参与水稻生长发育过程的控制，但乙烯在水稻中调节这些途径的机制尚不清楚。最近的研究发现，乙烯信号通路在水稻和拟南芥中是保守但不同的。正向遗传分析是全面揭示水稻乙烯信号传导机制的重要而有效的方法。在这里，我们提供了水稻乙烯反应突变体的遗传分析方案，包括乙烯反应突变体的筛选、乙烯和化学试剂的处理以及乙烯反应性基因表达分析。
[Abstract] Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ethylene-signaling pathway is conserved but different between rice and Arabidopsis. The forward genetic analysis is an essential and efficient method to reveal fully the mechanism of ethylene signaling in rice plants. Here we provide a protocol of genetic analysis of rice ethylene-response mutant, including screening ethylene-response mutants, treatment with ethylene and a chemical reagent, and ethylene-responsiveness gene expression analysis.