Elevated Levels of Activated and Pathogenic Eosinophils Characterize Moderate-Severe House Dust Mite Allergic Rhinitis.
Elevated Levels of Activated and Pathogenic Eosinophils Characterize Moderate-Severe House Dust Mite Allergic Rhinitis.
复制标题
活化和致病性嗜酸性粒细胞水平升高是中度至重度屋尘螨过敏性鼻炎的特征。
DOI：
10.1155/2020/8085615
复制
发表时间：
2020-01-01
影响因子：
4.1
通讯作者：
Sun, Yueqi
中科院分区：
文献类型：
--
作者：
Chen, Yang;Yang, Meng;Sun, Yueqi
嗜酸性粒细胞在变应性气道炎症的发病机制中起关键作用。然而，嗜酸性粒细胞活化和致病性在驱动变应性鼻炎（AR）疾病严重程度进展中的相对重要性仍有待确定。我们的目的是评估活化和致病性嗜酸性粒细胞与AR患者疾病严重程度的关系。从前瞻性招募的轻度（n = 10）和中重度（n = 21）屋尘螨AR患者和健康对照受试者（n = 10）中收集外周血和鼻样本。采用流式细胞仪检测血和鼻中嗜酸性粒细胞活化标志物和致病标志物的表达。比较轻、中、重度患者和健康对照者血嗜酸性粒细胞阳离子蛋白（ECP-）释放能力和Pro-Th 2功能。结果显示，中重度AR患者血和鼻中活化的（CD 44+和CD 69+）和致病的（CD 101 + CD 274+）嗜酸性粒细胞以及血嗜酸性粒细胞祖细胞的数量均高于轻度AR患者和健康对照组。此外，血液中活化和致病性嗜酸性粒细胞水平与AR患者的鼻部症状总评分、血清ECP和嗜酸性粒细胞过氧化物酶（EPX）水平呈正相关。中重度患者血嗜酸性粒细胞释放CCL 11诱导的ECP和EPX以及促进Th 2应答的能力高于轻度患者和健康对照。总之，中重度AR患者的特征是活化和致病性嗜酸性粒细胞水平升高，这与外周血中ECP、EPX和IL-4的较高产生相关。
Eosinophils play a critical role in the pathogenesis of allergic airway inflammation. However, the relative importance of eosinophil activation and pathogenicity in driving the progression of disease severity of allergic rhinitis (AR) remains to be defined. We aimed to assess the relation of activated and pathogenic eosinophils with disease severity of patients with AR. Peripheral blood and nasal samples were collected from patients with mild (n = 10) and moderate-severe (n = 21) house dust mite AR and healthy control subjects (n = 10) recruited prospectively. Expressions of activation and pathogenic markers on eosinophils in the blood and nose were analyzed by flow cytometry. The eosinophilic cation protein- (ECP-) releasing potential and the pro-Th2 function of blood eosinophils were compared between the mild and moderate-severe patients and healthy controls. Our results showed that the numbers of activated (CD44+ and CD69+) and pathogenic (CD101+CD274+) eosinophils in the blood and nose as well as blood eosinophil progenitors were increased in moderate-severe AR compared with the mild patients and healthy controls. In addition, the levels of activated and pathogenic eosinophils in the blood were positively correlated with the total nasal symptom score and serum ECP and eosinophil peroxidase (EPX) levels in patients with AR. Furthermore, the blood eosinophils obtained from the moderate-severe patients exhibited a higher potential of releasing ECP and EPX induced by CCL11 and of promoting Th2 responses than those from the mild patients and healthy controls. In conclusion, patients with moderate-severe AR are characterized by elevated levels of activated and pathogenic eosinophils, which are associated with higher production of ECP, EPX, and IL-4 in the peripheral blood.