Enhancement of leaf photosynthetic capacity through increased stomatal density in Arabidopsis
Enhancement of leaf photosynthetic capacity through increased stomatal density in Arabidopsis
复制标题
DOI：
10.1111/nph.12186
复制
发表时间：
2013-05-01
期刊：
影响因子：
9.4
通讯作者：
Hara-Nishimura, Ikuko
中科院分区：
文献类型：
--
作者：
Tanaka, Yu;Sugano, Shigeo S.;Hara-Nishimura, Ikuko
光合速率由CO2固定和CO2通过叶表皮中称为气孔的孔进入植物中决定。然而，气孔密度增加对光合速率的影响尚不清楚。本文研究了气孔密度变化对拟南芥叶片光合能力的影响。气孔密度调制过表达或沉默STOMAGEN，气孔发育的正调节。在转基因植物中检测叶片光合能力和植物生长。增加气孔密度在STOMAGEN过表达植物提高了30%的光合速率相比，野生型植物。转基因植物在环境CO2条件下表现出气孔导度的增加，并没有表现出改变的最大羧化速率，表明光合速率的增强是由气体扩散的变化。叶片光合作用-胞间CO2浓度响应曲线表明，在高CO2条件下，光合速率增加，气孔密度增加。STOMAGEN过表达并不改变整个植物的生物量，而其沉默引起生物量减少。我们的研究结果表明，增加气孔密度提高叶片光合能力，通过调节气体扩散。气孔密度可能是植物工程提高光合能力的一个目标性状。
Photosynthetic rate is determined by CO2 fixation and CO2 entry into the plant through pores in the leaf epidermis called stomata. However, the effect of increased stomatal density on photosynthetic rate remains unclear. This work investigated the effect of alteration of stomatal density on leaf photosynthetic capacity in Arabidopsis thaliana. Stomatal density was modulated by overexpressing or silencing STOMAGEN, a positive regulator of stomatal development. Leaf photosynthetic capacity and plant growth were examined in transgenic plants. Increased stomatal density in STOMAGEN-overexpressing plants enhanced the photosynthetic rate by 30% compared to wild-type plants. Transgenic plants showed increased stomatal conductance under ambient CO2 conditions and did not show alterations in the maximum rate of carboxylation, indicating that the enhancement of photosynthetic rate was caused by gas diffusion changes. A leaf photosynthesis-intercellular CO2 concentration response curve showed that photosynthetic rate was increased under high CO2 conditions in association with increased stomatal density. STOMAGEN overexpression did not alter whole plant biomass, whereas its silencing caused biomass reduction. Our results indicate that increased stomatal density enhanced leaf photosynthetic capacity by modulating gas diffusion. Stomatal density may be a target trait for plant engineering to improve photosynthetic capacity.