Intralobular Pulmonary Lymphatic Distribution in Normal Human Lung Using D2-40 Antipodoplanin Immunostaining
Intralobular Pulmonary Lymphatic Distribution in Normal Human Lung Using D2-40 Antipodoplanin Immunostaining
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DOI：
10.1369/jhc.2009.953067
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发表时间：
2009-07-01
影响因子：
3.2
通讯作者：
Bernaudin, Jean-Francois
中科院分区：
文献类型：
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作者：
Kambouchner, Marianne;Bernaudin, Jean-Francois
长期以来，人们一直认为，除了靠近呼吸性细支气管或其卫星动脉的有限区域外，肺小叶深处没有淋巴管存在的证据。使用D2-40单克隆抗体的免疫组织化学研究进行了正常肺标本从手术标本，特别注意淋巴管的小叶内分布。本研究表明，不仅在呼吸性细支气管周围的结缔组织中，而且还与小叶内小动脉和/或直径小于50 μ m的小静脉有关。在更远处的肺泡壁中也观察到直径为10 μ m至20 μ m的一些小叶间淋巴管。总之，本研究使用D2-40单克隆抗体证实了正常人肺小叶内存在小淋巴管，这些淋巴管从肺泡间小叶和构成肺泡旁小叶的小血管周围出现。这种细小的小叶内淋巴网络可能在多种肺泡和间质性肺疾病中起关键的病理生理作用，需要进一步研究。（J Histochern Cytochem 57：643-648，2009）
It has been assumed for a long time that except for limited areas close to respiratory bronchioles or their satellite arteries, there is no evidence of lymphatic vessels deep in the pulmonary lobule. An immunohistochemical study using the D2-40 monoclonal antibody was performed on normal pulmonary samples obtained from surgical specimens, with particular attention to the intralobular distribution of lymphatic vessels. This study demonstrated the presence of lymphatics not only in the connective tissue surrounding the respiratory bronchioles but also associated with intralobular arterioles and/or small veins even less than 50 mu m in diameter. A few interlobular lymphatic vessels with a diameter ranging from 10 mu m to 20 mu m were also observed further away, in interalveolar walls. In conclusion, this study, using the D2-40 monoclonal antibody, demonstrated the presence of small lymphatic channels within the normal human pulmonary lobules, emerging from interalveolar interstitium, and around small blood vessels constituting the paraalveolar lymphatics. This thin intralobular lymphatic network may play a key pathophysiological role in a wide variety of alveolar and interstitial lung diseases and requires further investigation. (J Histochern Cytochem 57:643-648, 2009)