Protective Effects of Polysaccharide Extracted from Portulacae oleracea L. on Colitis Induced by Dextran Sulfate Sodium
Protective Effects of Polysaccharide Extracted from Portulacae oleracea L. on Colitis Induced by Dextran Sulfate Sodium
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DOI：
10.1089/jmf.2019.4414
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发表时间：
2019-11-26
影响因子：
2.4
通讯作者：
Liu, Li
中科院分区：
文献类型：
--
作者：
Wang, Zheng;Liang, Yanni;Liu, Li
马齿仙多糖（Portulecoleracea L.，本研究制备了POLP，并观察了其对葡聚糖硫酸钠诱导的小鼠结肠炎的治疗作用。临床活动评分和H&E染色结果证实了POLP的治疗效果。POLP能减轻结肠炎小鼠的症状，改善结肠组织病理结构。测定四种细胞因子的表达水平。POLP处理可下调PGE（2）和IL-6水平。检测细胞考克斯-2蛋白表达水平和STAT 3磷酸化水平。结果表明，这两种蛋白在结肠炎时均升高，POLP治疗后均降低，表明这两种蛋白与POLP的保护作用密切相关。由于PGE（2）的合成是由考克斯-2催化的，而STAT 3的磷酸化可以诱导考克斯-2的表达，因此可以得出结论，STAT 3是与结肠炎中POLP发挥活性相关的关键蛋白。
Polysaccharide from Ma-chi-xian (Portulacae oleracea L., POLP) was prepared and the therapeutic effect on dextran sodium sulfate-induced colitis mice was investigated in this study. The results of clinical activity score and H&E staining confirmed the therapeutic effect of POLP. POLP could diminished the symptoms of colitis and improve colon histopathological structure of the colitis mice. The expression levels of four cytokines were determined. The concentrations of PGE(2) and IL-6 were downregulated by POLP treatment. The COX-2 protein expression levels and the STAT3 phosphorylation levels were detected. The results showed that these two protein levels were all increased in colitis and decreased after POLP treatment, indicating that these two proteins were closely related with the protective effect of POLP. Because the synthesis of PGE(2) is catalyzed by COX-2 and phosphorylation of STAT3 can induce the expression of COX-2, it was concluded that STAT3 was a key protein related to the POLP exerting its activity in colitis.