Urinary glycosaminoglycans and glycoproteins in a calcium oxalate crystallization system
Urinary glycosaminoglycans and glycoproteins in a calcium oxalate crystallization system
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DOI：
10.1016/j.carres.2011.09.022
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发表时间：
2012-01-10
影响因子：
3.1
通讯作者：
Gohel, Mayur Danny I.
中科院分区：
文献类型：
--
作者：
Poon, Ngork Wah;Gohel, Mayur Danny I.
本研究采用混合悬浮混合产物去除（MSMPR）体系测定了尿总糖胺聚糖（GAGs）、糖蛋白（GPs）和单个GAGs对草酸钙（CaOx）结晶成核速率（Bo）、生长速率(G)和悬浮密度（MT）的影响。在人工尿液（AU）中加入尿总GAGs、糖蛋白和单个GAGs，包括硫酸肝素（HS）、硫酸软骨素（CS）和透明质酸（HA），然后引入MSMPR试验箱，通过粒子计数器分析其晶体大小和数量。GAGs和GPs的加入对CaOx结晶的影响体现在基于晶体大小和晶体数计算的CaOx的Bo、G和MT等结晶指标的变化上。尿总gag对CaOx结晶影响无统计学意义。而单独测量时，HA、CS和HS等gag可增强Bo，抑制G。CS和HS增强了CaOx的MT，而HA对CaOx的MT无显著影响。尿总GPs显示晶体的G和MT增加。虽然尿中总GAGs对CaOx结晶的影响无统计学意义，但单个GAGs （CS、HS）通过增加较小晶体的悬浮密度促进CaOx结晶，提示结石风险降低，而HA对形成的CaOx MT无显著影响。泌尿系统全科医生提示增大的大小和MT提示较大的晶体和/或聚集体。(C) 2011 Elsevier Ltd.版权所有。
This study measures the effects of total urinary glycosaminoglycans (GAGs), glycoproteins (GPs) and individual GAGs on the nucleation rates (Bo), growth rates (G) and suspension densities (MT) of calcium oxalate (CaOx) crystallization by the mixed suspension mixed product removal (MSMPR) system. Total urinary GAGs, glycoproteins and individual GAGs including heparan sulfate (HS), chondroitin sulfate (CS) and Hyaluronic acid (HA) were added into the artificial urine (AU) and then introduced into the MSMPR test chamber and the crystal sizes and numbers were analyzed by a particle counter. The effects of added GAGs and GPs on CaOx crystallization were reflected by the changes on the crystallization indexes including the Bo, G and MT of CaOx that were calculated based on the crystal size and numbers. Total urinary GAGs showed no statistical significance on CaOx crystallization. However, individual GAGs such as HA, CS and HS enhanced Bo and suppressed the G when measured individually. CS and HS enhanced the MT while HA shown no significant change in the MT of CaOx. Total urinary GPs showed an increase in the G and MT of crystals. Although total urinary GAGs showed no statistically significant effect on CaOx crystallization, individual GAGs (CS, HS) promoted the CaOx crystallization by increasing the suspension density of smaller crystals, indicative of reduced risk of stones while HA showed no significance in the MT of CaOx formed. Urinary GPs indicated increased sizes and MT suggesting larger crystals and/or aggregates. (C) 2011 Elsevier Ltd. All rights reserved.