MULLER CELLS IN DETACHED HUMAN RETINA EXPRESS GLIAL FIBRILLARY ACIDIC PROTEIN AND VIMENTIN
MULLER CELLS IN DETACHED HUMAN RETINA EXPRESS GLIAL FIBRILLARY ACIDIC PROTEIN AND VIMENTIN
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DOI：
10.1007/bf00927264
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发表时间：
1990-01-01
影响因子：
2.7
通讯作者：
HONDA, Y
中科院分区：
文献类型：
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作者：
OKADA, M;MATSUMURA, M;HONDA, Y
我们使用免疫金方法研究了正常人视网膜和增殖性玻璃体视网膜病变 (PVR) 病例脱落人视网膜中 Muller 细胞内波形蛋白和神经胶质纤维酸性蛋白 (GFAP) 的表达。正常视网膜中的 Muller 细胞表现出波形蛋白免疫反应性和微弱的 GFAP 免疫反应性；然而，在脱离的视网膜中，它们表现出独特的 GFAP 免疫反应性以及波形蛋白免疫反应性。免疫电镜观察显示，正常视网膜的 Muller 细胞内的中间丝 (IF) 显示波形蛋白免疫反应性，而脱离视网膜的 Muller 细胞内的中间丝 (IF) 显示波形蛋白和 GFAP 免疫反应性。波形蛋白和 GFAP 的双重染色表明，在分离的视网膜中，在同一丝中观察到这两种蛋白质的免疫反应性。这些结果表明，在正常情况下，人Muller细胞的IF由波形蛋白组成，而脱离视网膜中的Muller细胞含有不同的IF，其由波形蛋白和GFAP组成。
We investigated the expression of vimentin and glial fibrillary acidic protein (GFAP) within Muller cells in normal human retinas and in detached human retinas of proliferative vitreoretinopathy (PVR) cases using the immunogold method. Muller cells in normal retinas showed vimentin immunoreactivity and faint GFAP immunoreactivity; however, in detached retinas they showed distinct GFAP immunoreactivity as well as vimentin immunoreactivity. Immunoelectron microscopic observation revealed that intermediate filaments (IF) within Muller cells in normal retinas showed vimentin immunoreactivity and that those within Muller cells in detached retinas showed both vimentin and GFAP immunoreactivity. Double staining for vimentin and GFAP showed that in detached retinas, these two protein immunoreactivities were observed in the same filaments. These results indicate that IF of human Muller cells consist of vimentin under normal conditions and that Muller cells in detached retinas contain different IF, which consist of vimentin and GFAP.