Mating can initiate stable RNA silencing that overcomes epigenetic recovery.
Mating can initiate stable RNA silencing that overcomes epigenetic recovery.
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DOI：
10.1038/s41467-021-24053-4
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发表时间：
2021-07-09
影响因子：
16.6
通讯作者：
Jose AM
中科院分区：
文献类型：
--
作者：
Devanapally S;Raman P;Chey M;Allgood S;Ettefa F;Diop M;Lin Y;Cho YE;Jose AM
稳定的表观遗传变化似乎并不常见，这表明这种变化通常会消失或得到修复。在几代人之间稳定地改变基因表达的变化可能需要目前未知的特定条件。在这里，我们报告了最小的顺式调控序列的组合可以支持在线虫交配时对单拷贝转基因及其衍生物的永久RNA沉默。交配破坏了相互竞争的基于RNA的机制，从而启动了可持续300代的沉默。这种稳定的沉默需要小RNA途径的组成部分，并可以沉默反式中的同源序列。虽然动物不能从交配诱导的沉默中恢复，但它们通常会从反式沉默中恢复并对其产生抵抗力。在大多数情况下，当双链RNA在驱动生殖系表达的不同调控环境中沉默相同的编码序列时，也可以观察到恢复。因此，我们提出，调节功能可以进化为反对对瞬时变化的永久性和潜在的适应不良反应。稳定的表观遗传学变化相对较少。在这里，作者报告了交配诱导线虫中的单拷贝转基因稳定沉默。这种稳定的沉默需要小RNA沉默的成分。
Stable epigenetic changes appear uncommon, suggesting that changes typically dissipate or are repaired. Changes that stably alter gene expression across generations presumably require particular conditions that are currently unknown. Here we report that a minimal combination of cis-regulatory sequences can support permanent RNA silencing of a single-copy transgene and its derivatives in C. elegans simply upon mating. Mating disrupts competing RNA-based mechanisms to initiate silencing that can last for >300 generations. This stable silencing requires components of the small RNA pathway and can silence homologous sequences in trans. While animals do not recover from mating-induced silencing, they often recover from and become resistant to trans silencing. Recovery is also observed in most cases when double-stranded RNA is used to silence the same coding sequence in different regulatory contexts that drive germline expression. Therefore, we propose that regulatory features can evolve to oppose permanent and potentially maladaptive responses to transient change. Stable epigenetic changes are relatively rare. Here the authors report that mating induces stable silencing of a single-copy transgene in C. elegans. Components of small RNA silencing are required for this stable silencing.
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影响因子：
64.5
作者：
Ashe A;Sapetschnig A;Weick EM;Mitchell J;Bagijn MP;Cording AC;Doebley AL;Goldstein LD;Lehrbach NJ;Le Pen J;Pintacuda G;Sakaguchi A;Sarkies P;Ahmed S;Miska EA
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影响因子：
64.5
作者：
Frøkjær-Jensen C;Jain N;Hansen L;Davis MW;Li Y;Zhao D;Rebora K;Millet JRM;Liu X;Kim SK;Dupuy D;Jorgensen EM;Fire AZ
通讯作者：
Fire AZ
DOI：
10.1126/science.1157647
发表时间：
2008-07-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Guang S;Bochner AF;Pavelec DM;Burkhart KB;Harding S;Lachowiec J;Kennedy S
通讯作者：
Kennedy S