Biomarkers of antioxidant capacity in the hydrophilic and lipophilic compartments of human plasma
Biomarkers of antioxidant capacity in the hydrophilic and lipophilic compartments of human plasma
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DOI：
10.1016/j.abb.2004.03.006
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发表时间：
2004-10-01
影响因子：
3.9
通讯作者：
Aldini, G
中科院分区：
文献类型：
--
作者：
Yeum, KJ;Russell, RM;Aldini, G
抗氧化剂位于血浆的亲水性和亲脂性隔室中，作为针对活性氧（ROS）的防御系统而积极参与，活性氧由于正常代谢和疾病而在体内持续产生。然而，当ROS的产生不受控制时，它会导致生物系统中的细胞脂质，蛋白质和DNA损伤。已经开发了几种测定血浆“总”抗氧化能力的方法，以研究氧化应激在病理条件下的参与，并评估膳食抗氧化剂的功能生物利用度。测定抗氧化能力的常规测定法主要测量血浆的水室中的抗氧化能力。因此，水溶性抗氧化剂，如抗坏血酸，尿酸和蛋白质硫醇主要影响这些测定，而脂溶性抗氧化剂，如生育酚和类胡萝卜素只起次要作用。然而，在血浆的亲水性和亲脂性隔室中的抗氧化剂之间存在积极的相互作用。因此，新的方法来定义“真正的”总抗氧化能力的血浆中应反映抗氧化网络之间的水溶性和脂溶性抗氧化剂在血浆中。揭示抗氧化剂的作用机制及其真正的抗氧化潜力将有助于我们优化体内的抗氧化防御。(C)2004年爱思唯尔公司All rights reserved.
Antioxidants located in both the hydrophilic and lipophilic compartments of plasma are actively involved as a defense system against reactive oxygen species (ROS), which are continuously generated in the body due to both normal metabolism and disease. However, when the production of ROS is not controlled, it leads to cellular lipid, protein, and DNA damage in biological systems. Several assays to measure 'total' antioxidant capacity of plasma have been developed to study the involvement of oxidative stress in pathological conditions and to evaluate the functional bioavailability of dietary antioxidants. Conventional assays to determine antioxidant capacity primarily measure the antioxidant capacity in the aqueous compartment of plasma. Consequently, water-soluble antioxidants such as ascorbic acid, uric acid and protein thiols mainly influence these assays, whereas fat-soluble antioxidants such as tocopherols and carotenoids play only a minor role. However, there are active interactions among antioxidants located in the hydrophilic and lipophilic compartments of plasma. Therefore, new approaches to define the 'true' total antioxidant capacity of plasma should reflect the antioxidant network between water- and fat-soluble antioxidants in plasma. Revelation of the mechanism of action of antioxidants and their true antioxidant potential will help us to optimize the antioxidant defenses in the body. (C) 2004 Elsevier Inc. All rights reserved.