Alternative splicing during Arabidopsis flower development results in constitutive and stage-regulated isoforms.
Alternative splicing during Arabidopsis flower development results in constitutive and stage-regulated isoforms.
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拟南芥花发育过程中的选择性剪接产生组成型和阶段调节的亚型。
DOI：
10.3389/fgene.2014.00025
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发表时间：
2014
影响因子：
3.7
通讯作者：
Ma H
中科院分区：
文献类型：
--
作者：
Wang H;You C;Chang F;Wang Y;Wang L;Qi J;Ma H
选择性剪接（alternative splicing，AS）是真核生物基因表达中的一个过程，多外显子基因的初级转录本被剪接成两个或多个不同的成熟转录本，从而增加蛋白质组的多样性。AS通常在不同组织或发育阶段之间差异调节。最近的研究表明，拟南芥中高达60%的含内含子基因发生AS。然而，人们对花发育过程中这一复杂而重要的过程知之甚少。为了研究选择性剪接基因不同亚型的优先表达，我们利用高通量RNA-Seq技术对拟南芥花发育3个阶段的转录组进行了分析，获得了不同AS事件的信息。我们确定了大约24，000个在这些阶段中的一个或多个阶段表达的基因，并发现近25%的多外显子基因具有两个或更多个剪接变体。这比以前报道的多器官和多阶段A型糖尿病的40-60%发生率要低。thaliana，表明在花发育中表达的许多基因以单一的优势异构体起作用。另一方面，1716个异构体在三个阶段之间差异表达，表明AS可能仍然在花发育的阶段转换中发挥重要作用。此外，还发现了337个新的转录区，其中大部分都是单个外显子。综上所述，我们的分析提供了一个全面的调查AS在花发育和促进进一步的基因组和遗传研究。
Alternative splicing (AS) is a process in eukaryotic gene expression, in which the primary transcript of a multi-exon gene is spliced into two or more different mature transcripts, thereby increasing proteome diversity. AS is often regulated differentially between different tissues or developmental stages. Recent studies suggested that up to 60% of intron-containing genes in Arabidopsis thaliana undergo AS. Yet little is known about this complicated and important process during floral development. To investigate the preferential expression of different isoforms of individual alternatively spliced genes, we used high throughput RNA-Seq technology to explore the transcriptomes of three floral development stages of Arabidopsis thaliana and obtained information of various AS events. We identified approximately 24,000 genes that were expressed at one or more of these stages, and found that nearly 25% of multi-exon genes had two or more spliced variants. This is less frequent than the previously reported 40–60% for multiple organs and stages of A. thaliana, indicating that many genes expressed in floral development function with a single predominant isoform. On the other hand, 1716 isoforms were differentially expressed between the three stages, suggesting that AS might still play important roles in stage transition during floral development. Moreover, 337 novel transcribed regions were identified and most of them have a single exon. Taken together, our analyses provide a comprehensive survey of AS in floral development and facilitate further genomic and genetic studies.
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