Full-length transcriptome sequencing from multiple tissues of duck, anas platyrhynchos
Full-length transcriptome sequencing from multiple tissues of duck, anas platyrhynchos
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鸭、绿头鸭多个组织的全长转录组测序
DOI：
10.1038/s41597-019-0293-1
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发表时间：
2019
期刊：
影响因子：
9.8
通讯作者：
Hou Zhuo-Cheng
中科院分区：
文献类型：
--
作者：
Yin ZhongTao;Zhang Fan;Smith Jacqueline;Kuo Richard;Hou Zhuo-Cheng
鸭（Anas platyrhynchos）是重要的经济水禽之一，是研究鸟类免疫保护机制的理想模型。不完整的鸭参考基因组和非常有限的全长cDNA的可用性，阻碍了识别的选择性剪接转录本，减缓了许多基础研究的鸭。我们将PacBio Iso-Seq技术应用于鸭的多种组织，用于转录组测序。我们获得了199，993份全长转录本，并对这些转录本进行了全面注释。从所有鉴定的转录本中预测了23，755个lncRNA，从3，346个基因中准确预测了35，031个选择性剪接事件，分为5个模型。我们的数据构成了一个大的增加，在已知数量的lncRNA，和选择性剪接的转录本鸭，并在改善目前的基因组注释中发挥了重要作用。此外，这些数据对其他鸟类的功能研究也非常有用。
Duck (Anas platyrhynchos), one of the most economically important waterfowl, is an ideal model for studying the immune protection mechanism of birds. An incomplete duck reference genome and very limited availability of full-length cDNAs has hindered the identification of alternatively spliced transcripts and slowed down many basic studies in ducks. We applied PacBio Iso-Seq technologies to multiple tissues from duck for use in transcriptome sequencing. We obtained 199,993 full-length transcripts and comprehensively annotated these transcripts. 23,755 lncRNAs were predicted from all identified transcripts and 35,031 alternative splicing events, which divided into 5 models, were accurately predicted from 3,346 genes. Our data constitute a large increase in the known number of both lncRNA, and alternatively spliced transcripts of duck and plays an important role in improving current genome annotation. In addition, the data will be extremely useful for functional studies in other birds.