A NOVEL CATIONIC LIPOSOME REAGENT FOR EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS
A NOVEL CATIONIC LIPOSOME REAGENT FOR EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS
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DOI：
10.1016/0006-291x(91)91366-k
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发表时间：
1991-08-30
影响因子：
3.1
通讯作者：
HUANG, L
中科院分区：
文献类型：
--
作者：
GAO, X;HUANG, L
合成了一种新型的胆固醇阳离子衍生物3β[N-（N′，N′-二甲氨基乙烷）-氨基甲酰基]胆固醇（DC-Chol），并将其用于二油基磷脂酰乙醇胺超声脂质体的制备。该新型阳离子脂质体试剂可高效转染A431人表皮样癌细胞、A549人肺癌细胞、L929小鼠成纤维细胞和YPT迷你猪原代内皮细胞。其活性高于商业试剂Lipofectin，并且在A431细胞中检测的毒性比Lipofectin低约4倍。该试剂易于合成，稳定性至少为6周。
A novel cationic derivative of cholesterol, 3β[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine. This novel cationic liposome reagent facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells, A549 human lung carcinoma cells, L929 mouse fibroblast cells, and YPT minipig primary endothelial cells. The activity was greater than that of a commercial reagent, Lipofectin, and was approximately 4-fold less toxic than Lipofectin when assayed with A431 cells. The reagent is easy to synthesize and stable for at least 6 weeks.