Changes of H+ pumps of tonoplast vesicle from wheat roots in vivo and in vitro under aluminum treatment and effect of calcium
Changes of H+ pumps of tonoplast vesicle from wheat roots in vivo and in vitro under aluminum treatment and effect of calcium
复制标题
铝处理下小麦根部液泡膜囊泡体内外H泵变化及钙的影响
DOI：
10.1080/01904169809365583
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发表时间：
1998
影响因子：
2.1
通讯作者：
You
中科院分区：
文献类型：
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作者：
Wenhua Zhang;Fenqin Zhang;Z. Shen;You
用0.1mM铝处理小麦幼苗7天。从根的顶端部分分离液泡膜囊泡，并测量H+-ATP酶和H+-PPase的活性。与对照组相比，0.1mM铝处理提高了H+-ATPase活性，降低了H+-PPase活性。高钙（Ca）（5 mM）的外部供应减弱了Al处理下H+-ATP酶活性的刺激程度，并缓解了H+-PPase活性的降低。0.1 mM Al处理后，H+-ATP酶活性降低，ATP的Vmax和Km值下降。用0.1 mM Al体外处理也降低了H+-PPase活性，但增加了PPi的Km。
Abstract Wheat seedlings were treated with 0.1 mM Al for 7 days. Tonoplast vesicles were isolated from apical segments of roots and activities of H+‐ATPase and H+‐PPase measured. Compared with the control, 0.1 mM Al increased H+‐ATPase activity and decreased H+‐PPase activity. High external supply of calcium (Ca) (5 mM) diminished the extent of the stimulation of H+‐ATPase activity and alleviated the reduction of H+‐PPase activity under Al treatment. However, 0.1 mM Al treatment in vitro resulted in the inhibition of H+‐ATPase activity with the decrease of Vmax and Km for ATP. In vitro treatment with 0.1 mM Al also decreased the H+‐PPase activity, but increased the Km for PPi.
松本 H.：植物和细胞生理学。
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