Repo-Man/PP1 regulates heterochromatin formation in interphase.
Repo-Man/PP1 regulates heterochromatin formation in interphase.
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DOI：
10.1038/ncomms14048
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发表时间：
2017-01-16
影响因子：
16.6
通讯作者：
Vagnarelli P
中科院分区：
文献类型：
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作者：
de Castro IJ;Budzak J;Di Giacinto ML;Ligammari L;Gokhan E;Spanos C;Moralli D;Richardson C;de Las Heras JI;Salatino S;Schirmer EC;Ullman KS;Bickmore WA;Green C;Rappsilber J;Lamble S;Goldberg MW;Vinciotti V;Vagnarelli P
Repo-Man是一种蛋白磷酸酶1（PP 1）靶向亚基，调节有丝分裂进程和染色质重塑。有丝分裂后，Repo-Man/PP 1仍然与染色质结合，但其在间期的功能尚不清楚。在这里，我们表明，Repo-Man，通过Nup 153，浓缩染色质在核周围和核孔篮的边缘富集。Repo-Man/PP 1调节异染色质的形成，使H3 S28去磷酸化，并且它对于异染色质蛋白1结合和H3 K27 me 3募集是必要且充分的。使用一种新的蛋白质基因组学方法，我们表明，Repo-Man是丰富的亚端粒区域与H2 AZ和H3.3和耗尽Repo-Man改变了这些区域的一个子集的外周定位和deliverates一些polycomb端粒基因的阻遏。这项研究显示了有丝分裂磷酸酶在调控表观遗传景观和间期基因表达中的作用。Repo-Man是蛋白磷酸酶1的染色体结合亚基，调节有丝分裂。在这里，de Castro和他的同事们表明Repo-Man也在间期调节异染色质，并调节基因抑制和染色质组织。
Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase. Repo-Man is a chromosome-binding subunit of protein phosphatase 1 to regulate mitosis. Here, de Castro and colleagues show that Repo-Man also regulates heterochromatin during interphase, and regulates gene repression and chromatin organization.