Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia
Plasma lipidomics reveal profound perturbation of glycerophospholipids, fatty acids, and sphingolipids in diet-induced hyperlipidemia
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DOI：
10.1016/j.cbi.2015.01.023
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发表时间：
2015-02-25
影响因子：
5.1
通讯作者：
Zhao, Ying-Yong
中科院分区：
文献类型：
--
作者：
Miao, Hua;Chen, Hua;Zhao, Ying-Yong
高血压是冠心病的主要危险因素，已成为一个重要的公共卫生问题。脂质组学是一种强大的技术，用于评估生物系统中的整体脂质代谢产物和发现生物标志物。在本研究中，高脂血症是通过喂养大鼠高脂饮食诱导的。采用超高效液相色谱-四极杆飞行时间synapt高分辨质谱联用技术测定血脂。采用正交偏最小二乘-判别分析、相关分析和热图分析等方法研究高脂血症大鼠的代谢变化。使用S-图检测潜在的生物标志物，并通过准确的质量数据、同位素模式和MSE片段信息进行鉴定。在饮食诱导的高脂血症大鼠中观察到显著升高的总胆固醇、甘油三酯和低密度脂蛋白胆固醇以及降低的高密度脂蛋白胆固醇。结合标准血清生化结果，观察到血浆脂质化合物包括11种甘油磷脂、6种脂肪酸、2种鞘脂、1种类花生酸、1种固醇脂质和1种甘油脂质的显著差异，突出了饮食诱导的高脂血症中脂质代谢的扰动。这些发现提供了进一步的见解，脂质分布在广泛的生化途径，饮食诱导的高脂血症。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Hyperlipidemia is a major risk factor for coronary heart disease and has emerged as an important public health problem. Lipidomics is a powerful technology for assessment of global lipid metabolites in a biological system and for biomarker discovery. In the present study, hyperlipidemia was induced by feeding rats a high fat diet. A sensitive ultra-performance liquid chromatography coupled with quadrupole time-of-flight synapt high-definition mass spectrometry method was used for the analysis of plasma lipids. Orthogonal partial least squares-discriminant analysis, correlation analysis and heatmap analysis were performed to investigate the metabolic changes in rats with diet-induced hyperlipidemia. Potential biomarkers were detected using S-plot and were identified by accurate mass data, isotopic pattern and MSE fragments information. Significantly increased total cholesterol, triglycerides and low-density lipoprotein cholesterol as well as decreased high-density lipoprotein cholesterol were observed in diet-induced hyperlipidemic rats. Combined with standard serum biochemical results, significant differences in plasma lipid compounds including eleven glycerophospholipids, six fatty acids, two sphingolipids, one eicosanoid, one sterol lipid and one glycerolipid were observed, highlighting the perturbation of lipid metabolism in diet-induced hyperlipidemia. These findings provide further insights into the lipid profile across a wide range of biochemical pathways in diet-induced hyperlipidemia. (C) 2015 Elsevier Ireland Ltd. All rights reserved.