The gene yggE functions in restoring physiological defects of Escherichia coli cultivated under oxidative stress conditions
The gene yggE functions in restoring physiological defects of Escherichia coli cultivated under oxidative stress conditions
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DOI：
10.1128/aem.71.5.2762-2765.2005
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发表时间：
2005-05-01
影响因子：
4.4
通讯作者：
Taya, M
中科院分区：
文献类型：
--
作者：
Kim, S;Nishioka, M;Taya, M
DNA微阵列分析表明，当超氧化物歧化酶（SOD）缺陷的大肠杆菌IM303（I4）在光激发TiO2产生的氧化应激下培养时，yfiD、yggB和yggE基因上调，并且通过将各自的基因插入pUC 19载体构建了pYFD、pYGB和pYGE。 pUC 19使携带pYGE的IM303(I4)细胞中活性氧(ROS)含量降低至对照细胞ROS含量的31%。在野生型菌株大肠杆菌MM294的培养中，在百草枯(10μmol/l)培养基中，携带pYGE的细胞的最大比生长率比对照细胞高约5倍，且前者细胞中ROS含量降低。 pYGE 的引入还抑制了在含有百草枯的 MM294 细胞培养物中氨基酸需求改变的细胞的出现。
DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO2, and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 vector. The content of reactive oxygen species (ROS) in IM303 (I4) cells carrying pYGE was reduced to 31% of ROS content in the control cells with pUC 19. In the culture of wild-type strain, E. coli MM294, in the medium with paraquat (10 mu mol/l), maximum specific growth rate of the cells with pYGE was about five times higher than that of the control cells, with a decreased ROS content in the former cells. The introduction of pYGE also suppressed the occurrence of the cells with altered amino acid requirement in the culture of MM294 cells with paraquat.