Prevalence of Malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in Brazil.
Prevalence of Malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in Brazil.
复制标题
马拉色菌的流行情况。
DOI：
10.1080/j.1365-280x.1999.00212.x
复制
发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
J. S. Hamdan
中科院分区：
文献类型：
--
作者：
E. R. Duarte;M. M. Melo;R. Hahn;J. S. Hamdan
马拉色菌属的酵母菌是亲脂微生物，是腐生菌，可以作为动物的机会致病菌。厚皮马拉色菌通常分离于健康犬的耳道和皮肤，或与脂溢性皮炎和外源性中耳炎有关。本研究的目的是确定马拉色菌在健康牛和患中耳炎牛耳中的发生情况。用无菌拭子收集标本（分泌物或耵聍），接种于Mycosel培养基，补充橄榄油，在35℃下孵育1周。根据形态特征、在32℃的Dixon培养基和添加Tween 20、40或80修饰的Sabouraud葡萄糖培养基中的生长情况对酵母进行鉴定。结果：中耳炎牛（75头）培养物阳性率为54.7%，健康牛（378头）培养物阳性率为34.6%。对来自中耳炎动物的阳性培养物（41）进行分析，可以推定鉴定出24种菌株，分别为球形m.b lobosa（12）、丝路m.s looffiae(5)、毛状m.s furfur(5)和联合m.s loopodialis(2)。对更多动物的进一步研究可能会证实迄今证实的趋势，即从中耳炎动物中分离出马拉色菌的频率高于从健康动物中分离出的频率（P<0.01），并且以全球马拉色菌为优势种。
Yeasts of the genus Malassezia are lipophilic microorganisms that are saprophytes that can act as opportunistic pathogens in animals. Malassezia pachydermatis is commonly isolated from the ear canal and skin of healthy dogs, or in association with seborrheic dermatitis and otitis externa conditions. The objective of the present study was to determine the occurrence of Malassezia spp. in the ears of healthy bovines and bovines with otitis. Specimens (secretion or cerumen) were collected with sterile swabs, inoculated onto Mycosel medium, supplemented with olive oil, and incubated at 35 degrees C for 1 week. Yeasts were identified according to morphological characteristics, growth in Dixon medium at 32 degrees C and Sabouraud glucose medium modified by the addition of Tween 20, 40 or 80. The results showed that 54.7% of the cultures were positive in bovines with otitis (75) and 34.6% were positive in healthy bovines (378). Analysis of the positive cultures (41) from animals with otitis allowed presumptive identification of 24 strains corresponding to M. globosa (12), M. slooffiae (5), M. furfur (5) and M. sympodialis (2). Further studies on a larger number of animals may confirm the trend verified thus far, i.e. a higher frequency of isolation of Malassezia spp. from animals with otitis than from healthy animals (P<0.01) and a predominance of the species M. globosa.