Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin
Soil Microbial Community Composition and Diversity Analysis under Different Land Use Patterns in Taojia River Basin
复制标题
洮家河盆地不同土地利用模式下土壤微生物群落组成及多样性分析
DOI：
10.3390/f14051004
复制
发表时间：
2023-05-12
期刊：
影响因子：
2.9
通讯作者：
Wang, Jun
中科院分区：
文献类型：
--
作者：
He, Zhe;Yuan, Chenglin;Wang, Jun
土壤微生物受其微环境的影响很大。为了揭示不同土地利用方式对土壤细菌和真菌群落组成和多样性的影响，采用16S rRNA、18S rRNA和高通量测序方法，分析了桃家河流域不同土地利用方式(菜地、荒地、林地、耕地)土壤细菌和真菌群落组成及多样性。利用Spearman分析和冗余度分析(RDA)研究了土壤理化性质与土壤细菌群落组成的相关性，构建了土壤理化性质与土壤细菌群落多样性的偏最小二乘通径模型。结果表明，菜地土壤细菌物种丰富度最高，荒地最低。变形杆菌为优势门(20.69%~32.70%)，放线菌为优势门(7.99%~16.95%)。林地真菌物种丰富度最高，耕地真菌物种丰富度最低。菜地、林地和耕地中真菌的优势门分别为毛霉门，分别占29.39%、41.36%和22.67%。子囊菌门(42.16%)是荒地的优势类群。子囊菌纲的菌丝菌是荒地和耕地中的优势类群。毛霉门的丝状真菌和球状真菌是菜地和林地的优势类群。Spearman分析结果表明，细菌和真菌群落中的优势类群与土壤pH、粘粒和沙子有显著的相关性(p<；0.01)。RDA结果表明，土壤粘粒、pH和水分是影响土壤微生物群落多样性的关键环境因子。与细菌相比，不同土地利用方式对真菌多样性的影响更大。这些结果为流域土壤微生物群落组成和多样性的变化提供了理论依据。
Soil microorganisms are greatly affected by their microenvironment. To reveal the influence of different land use patterns on the composition and diversity of soil bacterial and fungal communities, this study analyzed microbial (bacteria and fungi) community composition and diversity under different land use patterns (vegetable land, wasteland, woodland, cultivated land) based on 16S rRNA, 18S rRNA, and high-throughput sequencing method in the Taojia River Basin. Spearman analysis and redundancy analysis (RDA) were used to explore the correlation between soil physicochemical properties and soil fungal and bacterial community composition, and a partial least squares path model (PLS-PM) was constructed to express the causal relationship between soil physicochemical properties and soil bacterial and fungal community diversity. The results showed that the soil bacterial species richness was highest in vegetable land and the lowest in the wasteland. Proteobacteria is the dominant phylum (20.69%-32.70%), and Actinobacteria is the dominant class (7.99%-16.95%). The species richness of fungi in woodland was the highest, while was the lowest in cultivated land. The dominant phylum of fungi in vegetable land, woodland, and cultivated land is Mucoromycota, 29.39%, 41.36%, and 22.67%, respectively. Ascomycota (42.16%) is the dominant phylum in wasteland. Sordariomyetes of Ascomycota is the dominant class in wasteland and cultivated land. Mortierellomycetes and Glomeromycetes of Mucoromycota are the dominant class in vegetable land and woodland. The results of the Spearman analysis revealed that the dominant groups in the bacterial and fungal communities had significant correlations with soil pH, clay, and sand (p < 0.01). The RDA results showed that soil clay, pH, and moisture were the key environmental factors affecting the diversity of soil microbial communities. Fungal diversity is more affected by different land use patterns than bacteria. These results provided a theoretical basis for the changes in soil microbial community composition and diversity in river basins.